Spectroscopic studies of STZ-induced methylated-DNA in both in vivo and in vitro conditions
Spectroscopic studies of STZ-induced methylated-DNA in both in vivo and in vitro conditions
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DOI：
10.1016/j.saa.2008.02.002
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发表时间：
2008-12-01
影响因子：
4.4
通讯作者：
Khayatian, M.
中科院分区：
文献类型：
--
作者：
Bathaie, S. Z.;Sedghgoo, F.;Khayatian, M.
DNA加合物形成后的烷基化剂不仅对活细胞具有有害作用，而且会将这些有害信息传递给下一代。烷基化DNA的研究已经引入了不同的技术，其中大多数是专为研究特定目标DNA而设计的。但确切的差异之间的光谱和功能性质的烷基化DNA是没有见过的文献。本研究采用链脲佐菌素（STZ）体外和体内两种方法对DNA进行甲基化，然后采用各种技术对甲基化DNA进行研究。我们的研究结果表明：(1)与正常DNA相比，插入染料中的溴化乙锭的结合减少到甲基化DNA。(2)甲基化dna的CD光谱变化包括275 nm处正极的减少和从258nm交叉到更长的波长，这是由于存在额外的疏水甲基导致其周围的水减少引起的。(3)甲基化dna作为变性剂对DTAB的稳定性降低；(4)甲基化dna在电子转移反应中的类酶活性降低。总之，额外的甲基不仅会使DNA周围的水突出，而且还会导致氢键的丧失，构象的松动，阻止所需的相互作用，从而影响DNA的正常功能。(C) 2008 Elsevier B.V.版权所有
Alkylating agents after formation of DNA adduct not only posses their harmful role on living cells but also can transfer this information to the next generation. Different techniques have been introduced to study the alkylated DNA, most of which are specific and designed for investigation of specific target DNA. But the exact differences between spectroscopic and functional properties of alkylated DNA are not seen in the literature. In the present study DNA was methylated using streptozotocin (STZ) by both in vitro and in vivo protocols, then methylated-DNA was investigated by various techniques. Our results show that (1) the binding of ethidium bromide as,in intercalating dye decreases to methylated-DNA in comparison with normal DNA. (2) CD spectra of methylated-DNA show changes including a decrease in the positive hand at 275 nm and a shift from 258nm crossover to a longer wavelength, which is caused by reduction of water around it, due to the presence of additional hydrophobic methyl groups. (3) the stability of methylated-DNA against DTAB as a denaturant is decreased and (4) the enzyme-like activity of methylated-DNA in an electron transfer reaction is reduced. In conclusion, additional methyl groups not only protrude water around DNA, hut also Cause the loss of hydrogen bonding, loosening of conformation, preventing desired interactions and thus normal function of DNA. (C) 2008 Elsevier B.V. All rights reserved.