Protective Effects of Glycine soja Leaf and Stem Extract against Chondrocyte Inflammation and Osteoarthritis.
Protective Effects of Glycine soja Leaf and Stem Extract against Chondrocyte Inflammation and Osteoarthritis.
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DOI：
10.3390/ijms24054829
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发表时间：
2023-03-02
影响因子：
5.6
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中科院分区：
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野生大豆，又称野大豆（Glycine soja Sieb.）et Zucc. (GS)，一直以其各种健康益处而闻名。虽然G.大豆的GS叶和茎（GSLS）对骨关节炎（OA）的影响尚未得到评价。在这里，我们研究了GSLS在白细胞介素-1 β（IL-1β）刺激的SW 1353人软骨细胞中的抗炎作用。GSLS可抑制IL-1β刺激的软骨细胞中炎性细胞因子和基质金属蛋白酶的表达，并改善II型胶原的降解。GSLS通过抑制NF-κB的活化，对软骨细胞起到保护作用。此外，我们的体内研究表明，GSLS通过抑制碘乙酸（MIA）诱导的OA大鼠模型中的炎症反应来改善疼痛并逆转关节中的软骨退化。GSLS显著减轻MIA诱导的OA症状，如关节疼痛，并降低血清促炎介质、细胞因子和基质金属蛋白酶（MMPs）水平。我们的研究结果表明，GSLS发挥抗骨关节炎的作用，并减少疼痛和软骨退化，通过下调炎症，这表明它是一个有用的治疗OA的候选人。
Wild soybean, also known as Glycine soja Sieb. et Zucc. (GS), has long been known for its various health benefits. Although various pharmacological effects of G. soja have been studied, the effects of GS leaf and stem (GSLS) on osteoarthritis (OA) have not been evaluated. Here, we examined the anti-inflammatory effects of GSLS in interleukin-1β (IL-1β)-stimulated SW1353 human chondrocytes. GSLS inhibited the expression of inflammatory cytokines and matrix metalloproteinases and ameliorated the degradation of collagen type II in IL-1β-stimulated chondrocytes. Furthermore, GSLS played a protective role in chondrocytes by inhibiting the activation of NF-κB. In addition, our in vivo study demonstrated that GSLS ameliorated pain and reversed cartilage degeneration in joints by inhibiting inflammatory responses in a monosodium iodoacetate (MIA)-induced OA rat model. GSLS remarkably reduced the MIA-induced OA symptoms, such as joint pain, and decreased the serum levels of proinflammatory mediators, cytokines, and matrix metalloproteinases (MMPs). Our findings show that GSLS exerts anti-osteoarthritic effects and reduces pain and cartilage degeneration by downregulating inflammation, suggesting that it is a useful therapeutic candidate for OA.