Superparamagnetic iron oxide nanoparticles may affect endothelial progenitor cell migration ability and adhesion capacity.
Superparamagnetic iron oxide nanoparticles may affect endothelial progenitor cell migration ability and adhesion capacity.
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DOI：
10.3109/14653240903446910
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发表时间：
2010
期刊：
影响因子：
4.5
通讯作者：
Jinxiu Yang;Weiyu Tang;Xing-xiang Wang
中科院分区：
文献类型：
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作者：
Jinxiu Yang;Weiyu Tang;Xing-xiang Wang
用超顺磁性氧化铁（SPIO）纳米颗粒标记细胞使得能够非侵入性地跟踪移植细胞。本研究的目的是调查是否SPIO纳米粒子对内皮祖细胞（EPC）的功能活性的影响，并在优化的低剂量下使用SPIO纳米粒子标记猪和大鼠来源的EPC的方案的可行性。方法从猪外周血和大鼠骨髓中分离EPC并鉴定。在离体培养后，用SPIO纳米颗粒（以制备一系列终浓度，50、100、200和400 μ g/mL）或载体对照标记EPC。我们还研究了200 μ g/mL SPIO纳米颗粒对EPC的长期影响（标记后4、8、12和16天）。通过普鲁士蓝（PB）染色检测标记效率，并定量测定和证实细胞内铁摄取。分别采用MTT法和transwell小室法检测EPC增殖和迁移。EPC粘附测定通过将细胞重新铺板在纤连蛋白包被的培养皿上，然后计数粘附细胞来进行。使用Annexin V-FITC凋亡试剂盒评估EPC凋亡。结果SPIO纳米粒抑制EPC迁移，促进EPC粘附。EPC增殖和凋亡不受影响。SPIO纳米颗粒可以在200 μ g/mL下有效地标记EPC过夜，而不显著影响EPC功能活性。结论SPIO纳米粒可降低EPC的迁移能力，增强EPC的粘附能力。在合适的浓度（200 μ g/mL）下，EPC可被有效标记，而不显著影响其功能活性。
BACKGROUND AIMS Cell labeling with superparamagnetic iron oxide (SPIO) nanoparticles enables non-invasive tracking of transplanted cells. The aim of this study was to investigate whether SPIO nanoparticles have an effect on endothelial progenitor cell (EPC) functional activity and the feasibility of a protocol for labeling swine- and rat-origin EPC using SPIO nanoparticles at an optimized low dosage. METHODS EPC were isolated from the peripheral blood of swine and bone marrow of rat and characterized. After ex vivo cultivation, EPC were labeled with SPIO nanoparticles (to make a series of final concentrations, 50, 100, 200 and 400 microg/mL) or vehicle control. We also investigated the long-term effects of 200 microg/mL SPIO nanoparticles on EPC (4, 8, 12 and 16 days after labeling). The labeling efficiency was tested through Prussian blue (PB) staining and the intracellular iron uptake was also measured quantitatively and confirmed. EPC proliferation and migration were determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and transwell chamber assay, respectively. An EPC adhesion assay was performed by replating the cells on fibronectin-coated dishes and then counting the adherent cells. EPC apoptosis was evaluated using an Annexin V-FITC apoptosis kit. RESULTS SPIO nanoparticles impaired EPC migration and promoted EPC adhesion. EPC proliferation and apoptosis were not affected. SPIO nanoparticles could label EPC efficiently at 200 microg/mL overnight without significantly affecting EPC functional activity. CONCLUSIONS SPIO nanoparticles impaired the EPC migration ability and promoted the EPC adhesion capacity. EPC could be labeled efficiently at an appropriate concentration (200 microg/mL) without significantly affecting their functional activity.