REPLICATION OF THE B19 PARVOVIRUS IN HUMAN-BONE MARROW CELL-CULTURES
REPLICATION OF THE B19 PARVOVIRUS IN HUMAN-BONE MARROW CELL-CULTURES
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DOI：
10.1126/science.3738514
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发表时间：
1986-08-22
期刊：
影响因子：
56.9
通讯作者：
YOUNG, N
中科院分区：
文献类型：
--
作者：
OZAWA, K;KURTZMAN, G;YOUNG, N
B19 细小病毒至少导致三种人类疾病。该病毒在溶血性贫血患者的人红系骨髓悬浮培养物中成功繁殖；观察到新合成的病毒释放到受感染培养物的上清液中。该培养系统允许在分子水平上研究与 B19 生命周期相关的事件。 B19 细小病毒通过高分子量中间形式复制，并通过末端发夹结构连接。 B19 体外复制高度依赖于培养物的促红细胞生成含量和促红细胞生成素激素的添加。
The B19 parvovirus is responsible for at least three human diseases. The virus was successfully propagated in suspension cultures of human erythroid bone marrow from patients wiht hemolytic anemias; release of newly synthesized virus into the supernatants of infected cultures was observed. This culture system allowed study at a molecular level of events associated with the B19 life cycle. The B19 parvovirus replicated through high molecular weight intermediate forms, linked through a terminal hairpin structure. B19 replication in vitro was highly dependent on the erythropoietic content of cultures and on addition of the hormone erythropoietin.