ESCRT Machinery Is Required for Plasma Membrane Repair
ESCRT Machinery Is Required for Plasma Membrane Repair
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DOI：
10.1126/science.1247136
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发表时间：
2014-02-28
期刊：
影响因子：
56.9
通讯作者：
Perez, Franck
中科院分区：
文献类型：
--
作者：
Jimenez, Ana Joaquina;Maiuri, Paolo;Perez, Franck
质膜损伤可以由许多现象引发，有效的修复对细胞存活至关重要。胞吞作用、膜修补或细胞外出芽可用于质膜修复。我们发现，内体分选复合物所需的运输（ESCRT），以前参与膜出芽和分裂，在质膜修复中起着至关重要的作用。ESCRT蛋白在数秒内被募集到质膜伤口。结合数学建模的伤口闭合动力学定量分析表明，ESCRT参与了小伤口的修复。实时成像和相关扫描电子显微镜（SEM）确定了ESCRT募集部位的细胞外芽和脱落。因此，某些伤口的修复是通过受伤部分的ESCRT介导的细胞外脱落来确保的。
Plasma membrane damage can be triggered by numerous phenomena, and efficient repair is essential for cell survival. Endocytosis, membrane patching, or extracellular budding can be used for plasma membrane repair. We found that endosomal sorting complex required for transport (ESCRT), involved previously in membrane budding and fission, plays a critical role in plasma membrane repair. ESCRT proteins were recruited within seconds to plasma membrane wounds. Quantitative analysis of wound closure kinetics coupled to mathematical modeling suggested that ESCRTs are involved in the repair of small wounds. Real-time imaging and correlative scanning electron microscopy (SEM) identified extracellular buds and shedding at the site of ESCRT recruitment. Thus, the repair of certain wounds is ensured by ESCRT-mediated extracellular shedding of wounded portions.