Raphanus sativus extract protects against Zearalenone induced reproductive toxicity, oxidative stress and mutagenic alterations in male Balb/c mice
Raphanus sativus extract protects against Zearalenone induced reproductive toxicity, oxidative stress and mutagenic alterations in male Balb/c mice
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DOI：
10.1016/j.toxicon.2009.01.013
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发表时间：
2009-04-01
期刊：
影响因子：
2.8
通讯作者：
Oueslati, Ridha
中科院分区：
文献类型：
--
作者：
Ben Salah-Abbes, Jahla;Abbes, Samir;Oueslati, Ridha
玉米赤霉烯酮(Zen)是一种由谷物和农产品中的几种镰刀菌产生的非甾体类雌激素类真菌毒素。它与农场动物和人类的几种霉菌中毒有关。尽管作用机制尚不清楚，但有明确的证据表明，ZEN对雄性小鼠具有生殖毒性。一些报告表明，接触ZEN会导致氧化应激、遗传毒性和生殖参数的扰动。因此，本研究的目的是评价生长在突尼斯的萝卜水提物对ZEN诱导的生殖毒性和氧化应激的保护作用。将50只雄性Balb/c小鼠随机分为5组，分别为对照组、橄榄油处理组、ZEN组(40 mg/kg bw)、大蒜提取物(15 mg/kg bw)组和Zen+Rativus提取物组。采集睾丸组织标本，测定附睾精子数、睾酮浓度、丙二醛(MDA)水平、谷丙转氨酶(GPX)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性。采集血液样本进行不同的生化分析。同时，采用随机扩增-聚合酶链式反应(RAPD-PCR)的方法检测提取物在生殖细胞中的抗遗传毒性作用。结果表明，ZEN引起的毒理学效应与文献报道的一致：精子数量、睾酮水平和抗氧化酶状态下降。随机扩增-聚合酶链式反应分析显示，对照组和Zen处理组小鼠的DNA条带模式发生了变化。单独提取，富含许多抗氧化剂化合物，是安全的，成功地抵消了氧化应激，并防止了由ZEN引起的毒性。(C)2009爱思唯尔有限公司。保留所有权利。
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by several species of Fusarium in cereals and agricultural products. It has been implicated in several mycotoxicosis in farm animals and in humans. There is unequivocal evidence of reproductive toxicity of ZEN in male mice although the mechanism of action is unknown. Several reports suggest that exposure to ZEN resulted in oxidative stress, genotoxicity and perturbation of reproductive parameters. Therefore, the aim of the current study was to evaluate the protective effects of aqueous extract of Raphanus sativus growing in Tunisia against ZEN-induced reproductive toxicity and oxidative stress. Fifty male Balb/c mice were divided into five groups and treated for 28 days as follows: the control group, olive oil-treated groups, another treated with ZEN (40 mg/kg b.w), the last one treated with R. sativus extract alone (15 mg/kg b.w) and the other with ZEN + R. sativus extract. Testis samples were collected for the epididymal sperm count, testosterone concentration, and MDA level, GPx, CAT and SOD activities. Blood samples were collected for different biochemical analyses. Also, RAPD-PCR method was performed to assess the antigenotoxic effect of the extract in germ cells. The results indicated that ZEN-induced toxicological effects in accordance to those reported in the literature: decreasing in the sperm number, testosterone level and antioxidant enzyme status. The RAPD-PCR analysis revealed an alteration in the DNA bands patterns between control and ZEN-treated mice. The extract alone, rich in many antioxidant compounds, was safe and succeeded in counteracting the oxidative stress and protect against the toxicity resulting from ZEN. (C) 2009 Elsevier Ltd. All rights reserved.