Analysis of Cell Movement by Simultaneous Quantification of Local Membrane Displacement and Fluorescent Intensities Using Quimp2
Analysis of Cell Movement by Simultaneous Quantification of Local Membrane Displacement and Fluorescent Intensities Using Quimp2
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DOI：
10.1002/cm.20338
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发表时间：
2009-03-01
影响因子：
--
通讯作者：
Bretschneider, Till
中科院分区：
文献类型：
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作者：
Bosgraaf, Leonard;van Haastert, Peter J. M.;Bretschneider, Till
近年来，荧光标记在活细胞中的使用急剧增加。图像的定量分析需要特定的分析软件。此前，Quimp 程序启动用于定量细胞膜或皮层的荧光强度。然而，Quimp 不太适合定量局部膜位移。在这里，我们展示了 Quimp2，它能够及时跟踪膜分区，从而能够同时量化荧光强度和膜运动。 Quimp2 有两个新工具，(i) 转换滤镜，用于分析使用荧光、DIC 和相差不同显微镜获得的影片，以及 (ii) 一个宏，用于计算局部膜位移并提供各种选项来显示结果。 Quimp2 在此用于通过将局部膜运动的动力学与肌球蛋白和 F-肌动蛋白的局部浓度相关联来研究细胞运动的分子机制。细胞动力。细胞骨架 66：156-165，2009。 (C) 2009 Wiley-Liss, Inc.
The use of fluorescent markers in living cells has increased dramatically in the recent years. The quantitative analysis of the images requires specific analysis software. Previously, the program Quimp was launched for quantitating fluorescent intensities at the membrane or the cortex of the cell. However, Quimp is not well suited to quantitate local membrane displacement. Here we present Quimp2 that is capable of tracking membrane subregions in time, which enables the simultaneous quantification of fluorescent intensities and membrane movement. Quimp2 has two new tools, (i) conversion filters to analyze movies obtained with fluorescent, DIC and phase contrast different microscopes, and (ii) a macro that calculates the local membrane displacement and provides various options to display the results. Quimp2 is used here to investigate the molecular mechanism of cell movement by correlating the dynamics of local membrane movement with the local concentration of myosin and F-actin. Cell Motil. Cytoskeleton 66: 156-165,2009. (C) 2009 Wiley-Liss, Inc.