Simple and rapid process for single cell micro-patterning
Simple and rapid process for single cell micro-patterning
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DOI：
10.1039/b821581m
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Piel, Matthieu
中科院分区：
文献类型：
--
作者：
Azioune, Ammar;Storch, Marko;Piel, Matthieu
我们提出了一种简单而环保的方法，通过暴露于深紫外光，在覆盖有聚-L-赖氨酸-接枝-聚乙二醇（PLL-g-PEG）覆盖层的玻璃上进行细胞图案化。在与蛋白质孵育之前，图案化的基底在实验室气氛中稳定数月。与蛋白质一起孵育产生具有高特征分辨率的明确定义的图案。将接种在纤连蛋白/纤维蛋白原-Alexa 488图案上的RPE-1细胞在深UV暴露区域上限制数天。最后，以高均匀性对大玻璃板进行图案化，使得能够以96孔格式组装微图案化的微孔板。
We present a simple and environmentally friendly process for cell patterning on glass covered with an ultrathin layer of poly-L-lysine-grafted-polyethylene glycol (PLL-g-PEG) by exposure to deep UV light. The patterned substrates are stable for months in the lab atmosphere before incubation with proteins. Incubation with proteins resulted in well defined patterns, with high feature resolution. RPE-1 cells seeded on fibronectin/fibrinogen-Alexa 488 patterns were constrained for days on the deep UV exposed regions. Finally, large glass plates were patterned with high homogeneity enabling the assembly of micro-patterned microplates in 96-well format.