Identification of the interleukin-3 receptor using an iodinatable, cleavable, photoreactive cross-linking agent.
Identification of the interleukin-3 receptor using an iodinatable, cleavable, photoreactive cross-linking agent.
复制标题
使用可碘化、可裂解、光反应性交联剂鉴定 IL-3 受体。
DOI：
10.1016/s0021-9258(18)67620-3
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
G. Krystal
中科院分区：
文献类型：
--
作者：
P. Sorensen;N. Farber;G. Krystal
小鼠白细胞介素-3（mIL-3）是一种淋巴因子，其刺激多能造血干细胞及其定向子代的增殖和分化。然而，很少有人知道的机制，这种生长因子elevites其对响应细胞群体的影响。为了深入了解mIL-3受体相互作用后的早期事件，我们启动了分离受体并研究其特性的研究。在这份报告中，我们证明了使用一种新的碘化，裂解，光反应性交联剂，磺基琥珀酰亚胺基2-（对叠氮水杨酰胺基）-1，3 '-二硫代丙酸酯，以确定mIL-3受体。这些研究揭示了mIL-3受体是一条单链多肽链，分子量为67 kDa，等电点约为6.2。
Murine interleukin-3 (mIL-3) is a lymphokine that stimulates the proliferation and differentiation of both pluripotent hemopoietic stem cells and their committed progeny. However, very little is known about the mechanism by which this growth factor elicits its effects on responsive cell populations. To gain insight into early events following mIL-3 receptor interaction, we initiated studies to isolate the receptor and study its properties. In this report, we demonstrate the use of a new iodinatable, cleavable, photoreactive cross-linking agent, sulfosuccinimidyl 2-(p-azidosalicylamido)-1,3'-dithiopropionate to identify the mIL-3 receptor. These studies reveal the mIL-3 receptor to be a single polypeptide chain with a molecular weight of 67 kDa and an isoelectric point of approximately 6.2.