The insulinotrophic effect of insulin-like peptide 5 in vitro and in vivo
The insulinotrophic effect of insulin-like peptide 5 in vitro and in vivo
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DOI：
10.1042/bj20141113
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发表时间：
2015-03-15
影响因子：
4.1
通讯作者：
Wang, Ming-Wei
中科院分区：
文献类型：
--
作者：
Luo, Xiao;Li, Ting;Wang, Ming-Wei
胰岛素样肽5（INSL 5）是胰岛素/松弛素超家族的成员，可以激活G蛋白偶联受体松弛素/胰岛素样肽家族受体4（RXFP 4），但其确切的生物学功能在很大程度上是未知的。最近的研究表明，INSL 5/RXFP 4参与控制食物摄入和葡萄糖稳态。我们在本研究中报告，RXFP 4存在于小鼠胰岛素瘤细胞系MIN 6和INSL 5增强葡萄糖刺激的胰岛素分泌（GSIS）在体外和体内。RXFP 4也在小鼠肠L细胞系GLUTag中表达，INSL 5能够增强GLUTag细胞中葡萄糖依赖性胰高血糖素样肽-1（GLP-1）的分泌。我们认为INSL 5的促胰岛素分泌作用可能是通过刺激胰岛素/GLP-1分泌介导的，INSL 5/RXFP 4系统可能是2型糖尿病的潜在治疗靶点。
Insulin-like peptide 5 (INSL5), a member of the insulin/relaxin superfamily, can activate the G-protein-coupled receptor relaxin/insulin-like family peptide receptor 4 (RXFP4), but its precise biological functions are largely unknown. Recent studies suggest that INSL5/RXFP4 is involved in the control of food intake and glucose homoeostasis. We report in the present study that RXFP4 is present in the mouse insulinoma cell line MIN6 and INSL5 augments glucose-stimulated insulin secretion (GSIS) both in vitro and in vivo. RXFP4 is also expressed in the mouse intestinal L-cell line GLUTag and INSL5 is capable of potentiating glucose-dependent glucagon-like peptide-1 (GLP-1) secretion in GLUTag cells. We propose that the insulinotrophic effect of INSL5 is probably mediated through stimulation of insulin/GLP-1 secretion and the INSL5/RXFP4 system may be a potential therapeutic target for Type 2 diabetes.