Heparanase expression and localization in different types of human lung cancer
Heparanase expression and localization in different types of human lung cancer
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DOI：
10.1016/j.bbagen.2014.04.010
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发表时间：
2014-08-01
影响因子：
3
通讯作者：
Lima Castelo-Branco, Morgana Teixeira
中科院分区：
文献类型：
--
作者：
Fernandes dos Santos, Teresa Cristina;Gomes, Angelica Maciel;Lima Castelo-Branco, Morgana Teixeira
背景：乙酰肝素酶是唯一已知的能够裂解硫酸乙酰肝素链的哺乳动物糖苷酶。这种酶的表达与肿瘤的发展相关，因为它能够降解细胞外基质并促进细胞侵袭。方法：我们分析了肺癌样本中的乙酰肝素酶表达，以了解肺肿瘤的进展和恶性程度。在 37 名患者的样本中，有 14 名腺癌、13 名鳞状细胞癌、5 名大细胞癌和 5 名小细胞癌。进行免疫组织化学以确定乙酰肝素酶的表达和定位。结果：所有肿瘤类型均表达乙酰肝素酶，其主要定位于细胞质和细胞核内。在肿瘤微环境内的细胞中也观察到显着的酶表达，例如成纤维细胞、上皮细胞和炎症细胞。腺癌表现出最强的乙酰肝素酶染色强度和最广泛的乙酰肝素酶分布。鳞状细胞癌、大细胞癌和小细胞癌具有与腺癌相似的乙酰肝素酶亚细胞分布，但分布较不广泛。乙酰肝素酶表达往往与非小细胞肺癌的肿瘤淋巴结转移（TNM）分期相关。结论：在本研究中，我们发现乙酰肝素酶定位于肿瘤细胞的细胞质和细胞核以及不同类型肺癌微环境内的细胞。这种酶根据肺肿瘤的类型表现出不同的分布。一般意义：阐明不同类型肺癌中乙酰肝素酶的表达模式增加了我们对乙酰肝素酶在肺癌生物学中关键作用的理解。本文是题为“基质介导的细胞行为和特性”特刊的一部分。 (C) 2014 Elsevier B.V. 保留所有权利。
Background: Heparanase is the only known mammalian glycosidase capable of cleaving heparan sulfate chains. The expression of this enzyme has been associated with tumor development because of its ability to degrade extracellular matrix and promote cell invasion.Methods: We analyzed heparanase expression in lung cancer samples to understand lung tumor progression and malignancy. Of the samples from 37 patients, there were 14 adenocarcinomas, 13 squamous cell carcinomas, 5 large cell carcinomas, and 5 small cell carcinomas. Immunohistochemistry was performed to ascertain the expression and localization of heparanase.Results: All of the tumor types expressed heparanase, which was predominantly localized within the cytoplasm and nucleus. Significant enzyme expression was also observed in cells within the tumor microenvironment, such as fibroblasts, epithelial cells, and inflammatory cells. Adenocarcinomas exhibited the strongest heparanase staining intensity and the most widespread heparanase distribution. Squamous cell carcinomas, large cell carcinomas, and small cell carcinomas had a similar subcellular distribution of heparanase to adenocarcinomas but the distribution was less widespread. Heparanase expression tended to correlate with tumor node metastasis (TNM) staging in non-small cell lung carcinoma.Conclusion: In this study, we showed that heparanase was localized to the cytoplasm and nucleus of tumor cells and to cells within the microenvironment in different types of lung cancer. This enzyme exhibited a differential distribution based on the type of lung tumor. General significance: Elucidating the heparanase expression patterns in different types of lung cancer increased our understanding of the crucial role of heparanase in lung cancer biology. This article is part of a Special Issue entitled Matrix-mediated cell behaviour and properties. (C) 2014 Elsevier B.V. All rights reserved.