Joint degeneration following closed Intraarticular fracture in the mouse knee: A model of posttraumatic arthritis
Joint degeneration following closed Intraarticular fracture in the mouse knee: A model of posttraumatic arthritis
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DOI：
10.1002/jor.20331
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发表时间：
2007-05-01
影响因子：
2.8
通讯作者：
Olson, Steven A.
中科院分区：
文献类型：
--
作者：
Furman, Bridgette D.;Strand, Lens;Olson, Steven A.
创伤后关节炎是关节创伤后最常见的致残原因之一。本研究的目的是建立一种小鼠膝关节闭合性骨折模型，以量化创伤后关节炎模型中关节退变的时间顺序。使用计算机控制的材料测试系统和定制的压头在成年小鼠(C57BL/6)的胫骨平台上造成闭合性关节内骨折。使用高分辨率数字摄影对胫骨平台骨折进行分类并随时间推移进行成像。分别于骨折后2周、4周、8周和50周处死动物，取实验侧和对照侧肢体进行组织学和微CT分析。通过X线分析，胫骨平台骨折与临床骨折非常相似。更复杂和更粉碎的骨折与显著更高的骨折能量相关。组织学分析显示关节进行性退变，按改良的Markin分级，关节软骨中有纤颤和蛋白多糖丢失。在实验关节中也观察到软骨下骨增厚。小鼠胫骨平台闭合性关节内骨折的诱导产生了可复制的和临床相关的关节损伤，通过组织学和显微CT评估，该损伤进展为骨关节炎样改变。无需截骨术或开放关节切开术即可诱导关节退变的能力为研究创伤后关节炎的自然后遗症提供了一个有价值的新模型。值得注意的是，使用小鼠模型将有助于使用转基因动物来研究与创伤后关节炎的病理有关的特定基因。(C)2007年整形外科研究会。
Posttraumatic arthritis is one of the most frequent causes of disability following joint trauma. The objective of this study was to develop a model of a closed articular fracture in the mouse knee joint to quantify the temporal sequence of joint degeneration in a model of posttraumatic arthritis. Closed intraarticular fractures were created in the tibial plateau of adult mice (C57BL/6) using a computer-controlled materials testing system and a custom-built indenter tip. Tibial plateau fractures were classified and imaged over time using high-resolution digital radiography. Animals were sacrificed at 2, 4, 8, and 50 weeks following fracture, and the experimental and contralateral control limbs were harvested for histology and micro-computed tomography (microCT) analysis. By radiographic analysis, tibial plateau fractures closely resembled clinical fractures. More complex and comminuted fractures correlated to significantly higher fracture energies. Histologic analysis demonstrated progressive joint degeneration as measured by a modified Markin scale, with fibrillation and loss of proteoglycan in the articular cartilage. Subehondral bone thickening was also observed in experimental joints. The induction of a closed intraarticular fracture of the mouse tibial plateau generated a reproducible and clinically relevant joint injury that progressed to osteoarthritis-like changes by histologic and microCT evaluations. The ability to induce joint degeneration without an osteotomy or open arthrotomy provides a valuable new model for studying the natural sequelae of posttraumatic arthritis. Notably, the use ofa murine model will facilitate the use of genetically modified animals for the investigation ofspecific genes implicated in the pathology of posttraumatic arthritis. (c) 2007 Orthopaedic Research Society.