IDENTIFICATION OF COMMITTED MONONUCLEAR PRECURSORS FOR OSTEOCLAST-LIKE CELLS FORMED IN LONG-TERM HUMAN MARROW CULTURES
IDENTIFICATION OF COMMITTED MONONUCLEAR PRECURSORS FOR OSTEOCLAST-LIKE CELLS FORMED IN LONG-TERM HUMAN MARROW CULTURES
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DOI：
10.1210/endo-126-5-2733
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发表时间：
1990-05-01
期刊：
影响因子：
4.8
通讯作者：
ROODMAN, GD
中科院分区：
文献类型：
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作者：
KURIHARA, N;CHENU, C;ROODMAN, GD
将富含造血祖细胞的非贴壁骨髓单核细胞在含有重组人粒细胞巨噬细胞集落刺激因子的半固体培养基中培养9天，以形成集落形成单位粒细胞巨噬细胞（CFU-GM）集落。然后将 1,25-二羟基维生素 D 轻轻地铺在培养物上。两周后，大约 30% 形成的集落由具有独特多边形形态的细胞组成。这些集落中 100% 的多角形细胞与单克隆抗体 23c6 发生交叉反应，该抗体优先识别破骨细胞。这些多边形细胞的同质群体在悬浮培养中形成多核细胞 (MNC)，其中 100% 与 23c6 单克隆抗体发生交叉反应，并且超过 90% 的 MNC 响应降钙素而收缩。大约 20% 的 MNC 在钙化基质上形成吸收陷窝。这些结果表明 1) 早期破骨细胞前体源自 CFU-GM，即定型粒细胞-巨噬细胞祖细胞； 2) 定向单核破骨细胞前体具有独特的多边形形态，并与识别成熟破骨细胞的单克隆抗体发生交叉反应； 3)这些单核前体细胞能够形成满足破骨细胞功能标准的多核细胞。
Nonadherent marrow mononuclear cells enriched for hematopoietic progenitor cells were cultured in semisolid medium with recombinant human granulocyte-macrophage colony-stimulating factor for 9 days to form colony forming unit-granulocyte macrophage (CFU-GM) colonies. 1,25-Dihydroxy-vitamin D was then gently layered over the cultures. After 2 weeks, approximately 30% of the colonies that formed were composed of cells with a unique polygonal morphology. One hundred percent of the polygonal cells in these colonies cross-reacted with the monoclonal antibody 23c6, which preferentially recognizes osteoclasts. Homogenous populations of these polygonal cells formed multinucleated cells (MNC) in suspension culture, 100% of which cross-reacted with the 23c6 monoclonal antibody, and greater than 90% of the MNC contracted in response to calcitonin. Approximately 20% of these MNC formed resorption lacunae on calcified matrices. These results suggest that 1) early osteoclast precursors are derived from CFU-GM, the committed granulocyte-macrophage progenitor; 2) committed mononuclear osteoclast precursors have a distinct polygonal morphology and cross-react with monoclonal antibodies that recognize mature osteoclasts; and 3) these mononuclear precursors are capable of forming multinucleated cells which fulfill the functional criteria for osteoclasts.