Orthotopic and heterotopic autografts of frozen-thawed ovarian cortex in sheep
Orthotopic and heterotopic autografts of frozen-thawed ovarian cortex in sheep
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DOI：
10.1093/humrep/14.8.2149
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发表时间：
1999-08-01
影响因子：
6.1
通讯作者：
Driancourt, MA
中科院分区：
文献类型：
--
作者：
Aubard, Y;Piver, P;Driancourt, MA
冷冻卵巢皮质是一种新的选择，可以保存接受癌症治疗的年轻患者或面临过早更年期的女性的生育能力。然而，使用这些储存的组织的最佳方式仍不清楚。本研究比较了冻融绵羊卵巢皮质异位自体移植和原位自体移植的功能。将新鲜和冻融的卵巢皮质片自体移植到6只母羊的子宫角上(原位移植)和9只母羊的腹皮下移植(异位移植)。在原位和异位移植物中，监测卵泡生长和排卵的恢复。在原位移植的母羊中，记录了繁殖力。从两种类型的移植物中收集卵母细胞，在体外成熟和受精，新鲜移植物和冻融移植物的卵泡生长正常，分别在移植后4周和10周可检测到腔前卵泡和腔前卵泡，但仅有5%的原始卵泡存活。这证实了移植过程对卵泡存活的危害比冷冻更大，虽然大多数母羊恢复了排卵，但原位移植的母羊没有一只在同步交配时怀孕，移植7个月后，异位和原位移植的母羊都可以收集到卵母细胞，成熟并受精，但没有一只发育到囊胚期。如果移植物中的卵泡存活率明显提高，异位移植可能是一种替代原位移植以保持生育能力的方法。
Freezing ovarian cortex is a new option to preserve the fertility of young patients undergoing cancer treatment or in women facing premature menopause. However, the best way to use this banked tissue remains unclear. The function of heterotopic and orthotopic autografts of frozen-thawed ovarian cortex of sheep was compared in the present study. Fresh and frozen-thawed fragments of ovarian cortex were autografted on the uterine horn of six ewes (orthotopic grafts) and under the skin of the belly in nine ewes (heterotopic grafts). In both orthotopic and heterotopic grafts, the resumption of follicular growth and ovulation was monitored. In orthotopically grafted ewes, fertility was recorded. Oocytes from both types of grafts were collected, matured and fertilized in vitro, In both fresh and frozen-thawed grafts follicular growth resumed normally; preantral and antral follicles were first detectable 4 and 10 weeks respectively following grafting but only 5% of the primordial follicles appeared to have survived. This confirms that grafting procedures are more deleterious for follicle survival than cryopreservation, Although ovulation resumed in most ewes, none of the ewes grafted orthotopically became pregnant at a synchronized mating, Seven months following grafting, oocytes could be collected from heterotopic and orthotopic grafts, matured and some of them fertilized, but none developed to the blastocyst stage. Heterotopic grafting may be an alternative to orthotopic grafting to preserve fertility provided follicle survival in the grafts is markedly improved.