Two-photon laser scanning microscopy imaging of intact spinal cord and cerebral cortex reveals requirement for CXCR6 and neuroinflammation in immune cell infiltration of cortical injury sites.
Two-photon laser scanning microscopy imaging of intact spinal cord and cerebral cortex reveals requirement for CXCR6 and neuroinflammation in immune cell infiltration of cortical injury sites.
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DOI：
10.1016/j.jim.2009.09.007
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发表时间：
2010-01-31
影响因子：
2.2
通讯作者：
Dustin, Michael L.
中科院分区：
文献类型：
--
作者：
Kim, Jiyun V.;Jiang, Ning;Tadokoro, Carlos E.;Liu, Liping;Ransohoff, Richard M.;Lafaille, Juan J.;Dustin, Michael L.
小鼠脊髓是自身免疫和损伤模型的重要部位。颅骨减薄手术为小鼠大脑皮层的显微镜检查提供了一个微创窗口，但对于脊髓没有类似的方法。我们介绍了一种新的，简便和廉价的方法，双光子激光扫描显微镜的完整的脊髓在小鼠利用自然可达的椎间隙。当与基因靶向小鼠结合时，这些是强大的方法，其中内源性免疫细胞用绿色荧光蛋白（GFP）标记。我们首先证明了椎间窗的产生不会引起CX 3CR 1gfp/+小鼠中GFP+小胶质细胞的反应。我们接下来证明了在活动性实验性自身免疫性脑脊髓炎（EAE）期间CXCR 6 gfp/+小鼠的脊髓中GFP+免疫细胞的明显的喙尾向迁移。有趣的是，在这些小鼠中，GFP+细胞浸润大脑皮层需要三个条件：EAE诱导、皮层损伤和免疫细胞上CXCR 6的表达。
The mouse spinal cord is an important site for autoimmune and injury models. Skull thinning surgery provides a minimally invasive window for microscopy of the mouse cerebral cortex, but there are no parallel methods for the spinal cord. We introduce a novel, facile and inexpensive method for two-photon laser scanning microscopy of the intact spinal cord in the mouse by taking advantage of the naturally accessible intervertebral space. These are powerful methods when combined with gene-targeted mice in which endogenous immune cells are labeled with green fluorescent protein (GFP). We first demonstrate that generation of the intervertebral window does not elicit a reaction of GFP+ microglial cells in CX3CR1gfp/+ mice. We next demonstrate a distinct rostro-caudal migration of GFP+ immune cells in the spinal cord of CXCR6gfp/+ mice during active experimental autoimmune encephalomyelitis (EAE). Interestingly, infiltration of the cerebral cortex by GFP+ cells in these mice required three conditions: EAE induction, cortical injury and expression of CXCR6 on immune cells.
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脊髓细胞细胞募集会导致急性病毒性脑膜炎期间致命的中枢神经系统损伤。
影响因子：
64.8
作者：
Kim, Jiyun V.;Kang, Silvia S.;Dustin, Michael L.;McGavern, Dorian B.
通讯作者：
McGavern, Dorian B.
影响因子：
9.2
作者：
Loetscher, M;Amara, A;Moser, B
通讯作者：
Moser, B
影响因子：
4.4
作者：
Fukumoto, N;Shimaoka, T;Yonehara, S
通讯作者：
Yonehara, S
影响因子：
4.4
作者：
Huang, Julie H.;Cardenas-Navia, L. Isabel;Dustin, Michael L.
通讯作者：
Dustin, Michael L.
影响因子：
5.3
作者：
Jung, S;Aliberti, J;Littman, DR
通讯作者：
Littman, DR