Comparison of the Acute Effects of anti-TNF-alpha Drugs on a Uveitis Experimental Model
Comparison of the Acute Effects of anti-TNF-alpha Drugs on a Uveitis Experimental Model
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DOI：
10.3109/09273940903521964
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发表时间：
2010-06-01
影响因子：
3.3
通讯作者：
Javier Romero, Francisco
中科院分区：
文献类型：
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作者：
Johnsen-Soriano, Siv;Sancho-Tello, Maria;Javier Romero, Francisco
目的：比较抗TNF-α药物阿达木单抗和英夫利西单抗在葡萄膜炎实验模型中的组织病理学和生化作用。（200 μ g进入足垫）和给药，以及对房水中氧化应激相关标志物的生化分析。内毒素作用24 h后，大鼠眼前房出现严重炎症反应。只有英夫利西单抗给药部分阻止了内毒素诱导的血-房水屏障破坏，以及房水中Rantes和MCP-1浓度的增加。两种药物均改善了内毒素后的组织病理学评分。生化分析表明，这两种药物对内毒素诱导的氧化应激保护，恢复所有标志物的控制水平，除了英夫利西单抗，未能恢复GSH concentration.Conclusions：这两种抗TNF-α药物是有效的，在减少组织病理学炎症，但他们的作用机制似乎是不同的。
Purpose: To compare histopathological and biochemical effects of the anti-TNF-alpha drugs adalimumab and infliximab in a uveitis experimental model.Methods: Histopathological evaluation was performed 24 h after endotoxin (200 mu g into the footpad) and drug administration, as well as biochemical analysis of oxidative stress-related markers in the aqueous humor.Results: Severe inflammation was found in rat anterior chamber of the eye 24 h after endotoxin. Only infliximab administration partially prevented the endotoxin-induced disruption of the blood-aqueous barrier, as well as the increase in Rantes and MCP-1 concentration in aqueous humor. Both drugs ameliorated the histopathological score after endotoxin. Biochemical analysis revealed that both drugs protected against endotoxin-induced oxidative stress, restoring all markers to control levels, except infliximab, which failed to restore GSH concentration.Conclusions: Both anti-TNF-alpha drugs were effective in reducing histopathological inflammation but their mechanism of action appears to be different.