Important roles of the hyphenated HPLC‐DAD‐MS‐SPE‐NMR technique in metabonomics
Important roles of the hyphenated HPLC‐DAD‐MS‐SPE‐NMR technique in metabonomics
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DOI：
10.1002/mrc.2513
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发表时间：
2009-12
影响因子：
2
通讯作者：
Huiru Tang;Chaoni Xiao;Yulan Wang
中科院分区：
文献类型：
--
作者：
Huiru Tang;Chaoni Xiao;Yulan Wang
代谢物鉴定是代谢组学研究的关键步骤。全自动联用的高效液相-二极管阵列检测器(DAD)-质谱仪(MS)固相萃取(SPE)-核磁共振谱(HPLC-DAD-MS-SPE-核磁共振)是确定复杂混合物(代谢组)中未知代谢物结构的最有效的方法之一，因此是进一步发展代谢组学最重要的分析技术之一。本文综述了该技术在鉴定植物提取物中新的微量代谢物和药物代谢方面的最新应用。这种联用技术的改进，使强极性代谢物能够多重捕获生物体液，需要进一步的开发。版权所有©2009 John Wiley&Sons，Ltd.
Metabolite identification is a key step for metabonomics study. A fully automated hyphenation of HPLC‐diode‐array detector (DAD) mass spectrometry (MS) solid phase extraction (SPE)–NMR spectroscopy (HPLC‐DAD‐MS‐SPE‐NMR) is one of the most efficient methods to determine the structure of a given unknown metabolite in a complex mixture (metabonome) and hence represents one of the most important analytical techniques for the further development of metabonomics. In this review, some recent applications of this technique in identifying novel and trace metabolites in plant extracts and drug metabolism have been discussed. Modification of this hyphenated technique, enabling multiple trappings of strong polar metabolites for biofluids, needs further development. Copyright © 2009 John Wiley & Sons, Ltd.