STIMULATION OF DIFFERENTIATION AND PROLIFERATION OF HEMOPOIETIC CELLS IN-VITRO
STIMULATION OF DIFFERENTIATION AND PROLIFERATION OF HEMOPOIETIC CELLS IN-VITRO
复制标题
DOI：
10.1002/jcp.1040820315
复制
发表时间：
1973-01-01
影响因子：
5.6
通讯作者：
LORD, BI
中科院分区：
文献类型：
--
作者：
DEXTER, TM;ALLEN, TD;LORD, BI
一种用于造血细胞的液体培养系统已经开发出来，该系统使用骨髓细胞单独或胸腺和骨髓细胞共同培养，接种于4盎司的药瓶中。经过几天的生长，这种培养包括附着的细胞群体，形成离散的菌落，和非附着的群体。在（共培养）中，与（单独骨髓）和（单独胸腺）培养的总和相比，单层菌落的发育增强了2倍。此外，在共培养中，非附着细胞得到了更好的维护。正常的cff和cff在（单独骨髓）和（共培养）中至少存在14天。在（单独骨髓）培养中，在第一周，所有发育阶段的粒细胞都存在，但到第12天，培养主要由单核细胞组成。然而，在共培养组中，在12天的所有分化阶段，超过60%的细胞是粒细胞。使用致命辐射胸腺细胞建立的（共培养）不能支持这种延长的髓细胞分化。通过喂食（共培养物），可以维持（粒细胞）细胞的产生至少十周，尽管没有观察到完全成熟的粒细胞。第二次饲喂后，没有检测到CFUC，但至少十周内存在不同数量的琼脂集落形成细胞（不是经典的CFUC）。
A liquid culture system, for haemopoietic cells, has been developed using bone marrow cells alone, or co‐cultures of thymus and bone marrow cells, inoculated into four ounce medical bottles. After several days growth, such cultures consisted of an attaching population of cells, forming discrete colonies, and a non‐attaching population. In the (co‐cultures) there was a 2 X enhancement of monolayer colony development compared with the combined total present in the (marrow alone) plus (thymus alone) cultures. Also, better maintenance of non‐attaching cells was seen in the (co‐cultures). Normal CFUSand CFUCwere present in both the (marrow alone) and the (co‐cultures) for at least 14 days.In the (marrow alone) cultures, granulocytes in all stages of development were present for the first week, but by 12 days the culture consisted mainly of mono‐nuclear cells. In the (co‐cultures), however, at 12 days more than 60% of the cells were granulocytes, in all stages of differentiation. (Co‐cultures) established using lethally irradiated thymus cells were not able to support this prolonged myeloid differentiation.By feeding the (co‐cultures) it was possible to maintain production of (granulocytic) cells for at least ten weeks, although no fully mature granulocytes were observed. After the second feeding, no CFUSwere detectable, but variable numbers of agar colony forming cells (not classical CFUC) were present at least for ten weeks.