Loss and fragmentation of chromosome 5 are major events linked to the adaptation of rad52-ΔΔ strains of Candida albicans to sorbose
Loss and fragmentation of chromosome 5 are major events linked to the adaptation of rad52-ΔΔ strains of Candida albicans to sorbose
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DOI：
10.1016/j.fgb.2007.01.005
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发表时间：
2007-08-01
影响因子：
3
通讯作者：
Larriba, German
中科院分区：
文献类型：
--
作者：
Andaluz, Encarnacion;Gomez-Raja, Jonathan;Larriba, German
白色念珠菌可以通过丢失一份 Chr5 拷贝来适应山梨糖平板并在其上生长。由于酿酒酵母rad52突变体染色体丢失率很高，我们研究了白色念珠菌rad52对山梨糖的适应能力。 Carad52-Delta Delta 突变体比野生型更早产生 Sou(+) 菌株，但最终产量较低，可能是因为它们在山梨糖中的死亡率较高。与白色念珠菌的其他菌株一样，CAF2 和 rad52-Delta Delta 衍生物在大约 75% 的情况下会通过丢失一个 Chr5 拷贝而产生 Sou(+) 菌株。此外，rad52菌株能够通过Chr5的一个拷贝中的片段化/缺失事件产生Sou(+)菌株，该片段化/缺失事件包括与右侧端粒相邻的区域的丢失。最后，CAF2 和 rad52-Delta Delta 都产生了具有两个明显完整 Chr5 拷贝的 Sou(+) 菌株，这表明额外的基因组变化也可能调节对山梨糖的适应。 (c) 2007 Elsevier Inc. 保留所有权利。
Candida albicans can adapt and grow on sorbose plates by losing one copy of Chr5. Since rad52 mutants of Saccharomyces cerevisiae lose chromosomes at a high rate, we have investigated the ability of C albicans rad52 to adapt to sorbose. Carad52-Delta Delta mutants generate Sou(+) strains earlier than wild-type but the final yield is lower, probably because they die at a higher rate in sorbose. As other strains of C albicans, CAF2 and rad52-Delta Delta derivatives generate Sou(+) strains by a loss of one copy of Chr5 about 75% of the time. In addition, rad52 strains were able to produce Sou(+) strains by a fragmentation/deletion event in one copy of Chr5, consisting of loss of a region adjacent to the right telomere. Finally, both CAF2 and rad52-Delta Delta produced Sou(+) strains with two apparent full copies of Chr5, suggesting that additional genomic changes may also regulate adaptation to sorbose. (c) 2007 Elsevier Inc. All rights reserved.