Fractionation of Stable Zinc Isotopes in the Zinc Hyperaccumulator Arabidopsis halleri and Nonaccumulator Arabidopsis petraea
Fractionation of Stable Zinc Isotopes in the Zinc Hyperaccumulator Arabidopsis halleri and Nonaccumulator Arabidopsis petraea
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DOI：
10.1021/es200874x
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发表时间：
2011-11-01
影响因子：
11.4
通讯作者：
Oger, P.
中科院分区：
文献类型：
--
作者：
Aucour, A. M.;Pichat, S.;Oger, P.
锌同位素分馏可能为植物锌的吸收、运输和储存机制提供新的认识。本文研究了锌超积累植物拟南芥和非积累植物A.佩特拉植物在水培条件下生长，可测定源Zn（以Zn（2+）为主要形态）、地上部和根之间的同位素分馏。Zn同位素质量平衡结果表明，植物和源Zn δ（66）Zn（源内）的平均同位素分馏在非富集区为-0.19 +/-0.20ppm，在超富集区为-0.05 +/-0.12ppm。地上部Zn和掺入的大量Zn之间的同位素分馏（Delta（66）Zn（shoot-in））在非积累植物和超积累植物之间不同，并且是根-茎转运的函数（如由地上部Zn和大量植物Zn之间的质量比给出）。与根中螯合相关的大的同位素分馏（0.37份/千）指向Zn（2+）与根细胞中的高亲和力配体的结合。我们的结论是，锌稳定同位素可能有助于估计地下和空中锌储存在植物中，并在研究细胞外和细胞内的根螯合机制是有用的。
Zn isotope fractionation may provide new insights into Zn uptake, transport and storage mechanisms in plants. It was investigated here in the Zn hyperaccumulator Arabidopsis halleri and the nonaccumulator A. petraea. Plant growth on hydroponic solution allowed us to measure the isotope fractionation between source Zn (with Zn(2+) as dominant form), shoot and root. Zn isotope mass balance yields mean isotope fractionation between plant and source Zn Delta(66)Zn(in-source) of -0.19 +/- 0.20 parts per thousand in the nonaccumulator and of -0.05 +/- 0.12 parts per thousand in-source in the hyperaccurnulator. The isotope fractionation between shoot Zn and bulk Zn incorporated (Delta(66)Zn(shoot-in)) differs between the nonaccumulator and the hyperaccumulator and is function of root-shoot translocation (as given by mass ratio between shoot Zn and bulk plant Zn). The large isotope fractionation associated with sequestration in the root (0.37 parts per thousand) points to the binding of Zn(2+) with a high affinity ligand in the root cell. We conclude that Zn stable isotopes may help to estimate underground and aerial Zn storage in plants and be useful in studying extracellular and cellular mechanisms of sequestration in the root.