Diversity of Streptococcus mutans strains in bacterial interspecies interactions
Diversity of Streptococcus mutans strains in bacterial interspecies interactions
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DOI：
10.1002/jobm.201200457
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发表时间：
2014-02-01
影响因子：
3.1
通讯作者：
Deng, Dong Mei
中科院分区：
文献类型：
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作者：
Li, Xiaolan;Hoogenkamp, Michel A.;Deng, Dong Mei
生物被膜是由基质包裹的微生物群体相互粘附和粘附在表面上。与嗜热细菌细胞相比，生物膜细胞显示出高得多的抗微生物剂抗性水平。本研究旨在探讨变形链球菌生物被膜形成的多样性及单株变形链球菌对洗必泰的耐药性。mutans和双重S.突变体-粪肠球菌生物膜。四个临床S.变异株（C180-2、C67-1、HG 723和UA 159）在有或没有大肠杆菌的情况下形成24小时生物膜。粪菌株这些生物膜用0.025%CHX处理10分钟。生物膜形成、CHX抗性和变异链球菌-E.通过生物量染色、刃天青代谢、活菌计数和竞争琼脂测定来评价粪便相互作用。主要发现是，E.粪肠球菌一般减少所有双物种生物膜的形成，但比例的S。双菌种生物膜中的变形杆菌以及CHX抗性显示出明显的S.变异株依赖性特别地，在双S中观察到对CHX的抗性降低。变形链球菌C67-1生物膜，而增加的阻力，发现在双S。变形杆菌UA 159生物膜。总之，S.变异株E.生物膜中的粪便因菌株而异，这强调了研究菌株多样性在种间毒力调节和生物膜抗菌药物耐药性中的重要性。
Biofilms are matrix-enclosed microbial population adhere to each other and to surfaces. Compared to planktonic bacterial cells, biofilm cells show much higher levels of antimicrobial resistance. We aimed to investigate Streptococcus mutans strain diversity in biofilm formation and chlorhexidine (CHX) resistance of single S. mutans and dual S. mutans-Enterococcus faecalis biofilms. Four clinical S. mutans strains (C180-2, C67-1, HG723 and UA159) formed 24-h biofilms with or without an E. faecalis strain. These biofilms were treated for 10min with 0.025% CHX. Biofilm formation, CHX resistance and S.mutans-E. faecalis interactions were evaluated by biomass staining, resazurin metabolism, viable count and competition agar assays. The main finding is that the presence of E. faecalis generally reduced all dual-species biofilm formation, but the proportions of S. mutans in the dual-species biofilms as well as CHX resistance displayed a clear S. mutans strain dependence. In particular, decreased resistance against CHX was observed in dual S. mutans C67-1 biofilms, while increased resistance was found in dual S. mutans UA159 biofilms. In conclusion, the interaction of S. mutans with E. faecalis in biofilms varies between strains, which underlines the importance of studying strain diversity in inter-species virulence modulation and biofilm antimicrobial resistance.