Trends and molecular mechanisms of antimicrobial resistance in clinical staphylococci isolated from companion animals over a 16 year period
Trends and molecular mechanisms of antimicrobial resistance in clinical staphylococci isolated from companion animals over a 16 year period
复制标题
DOI：
10.1093/jac/dkw029
复制
发表时间：
2016-06-01
影响因子：
5.2
通讯作者：
Pomba, Constanca
中科院分区：
文献类型：
--
作者：
Couto, Natacha;Monchique, Claudia;Pomba, Constanca
目的：本研究的目的是探讨葡萄球菌对抗菌药物的耐药性演变、耐药机制及分子特征，方法：对1999 - 2014年从犬、猫、马等动物分离的632株临床葡萄球菌进行38种抗菌药物的药敏试验。采用PCR方法筛选出mecA、mecC等20个耐药基因。采用spa（金黄色葡萄球菌）、SCCmec、MLST和PFGE分型对耐甲氧西林葡萄球菌进行鉴定。使用SAS v9.3进行统计分析，如果P
Objectives: The objective of this study was to investigate the evolution of resistance to antimicrobials, corresponding mechanisms and molecular characteristics of Staphylococcus spp., between 1999 and 2014.Methods: Susceptibility to 38 antimicrobials was determined for 632 clinical staphylococcal isolates obtained from companion animals (dogs, cats, horses and other animals). Twenty antimicrobial resistance genes, including mecA and mecC, were screened by PCR. Methicillin-resistant staphylococci were characterized by spa (Staphylococcus aureus), SCCmec, MLST and PFGE typing. Statistical analyses were performed using SAS v9.3 and differences were considered relevant if P