Development of recombinant capsid antigen/transmembrane epitope fusion proteins for serological diagnosis of animal lentivirus infections
Development of recombinant capsid antigen/transmembrane epitope fusion proteins for serological diagnosis of animal lentivirus infections
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DOI：
10.1016/j.jviromet.2004.06.001
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发表时间：
2004-10-01
影响因子：
3.1
通讯作者：
Ciabatti, IM
中科院分区：
文献类型：
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作者：
Rosati, S;Profiti, M;Ciabatti, IM
在动物慢病毒中，猫免疫缺陷病毒（FIV）、马传染性贫血病毒（EIAV）和小反刍动物慢病毒（SRLV）是与免疫缺陷、贫血、关节炎、肺炎等多种临床症状相关的重要病原体。病毒抗体应答的检测代表了所有慢病毒感染中的实用诊断方法，因为它们保持可检测的长寿命。衣壳抗原（Capsidantigen，CA）是病毒的主要核心蛋白，抗CA的特异性抗体通常首先在感染的绵羊、山羊和马中被识别，并在很长一段时间内保持可检测性。包膜糖蛋白的跨膜（Transmembrane，TM）结构域包含已知在几种慢病毒中形成免疫显性表位的高度保守的基序。在这项研究中，开发了一种简单的策略，表达整个CA和TM表位在一个单一的融合蛋白从马，猫和小反刍动物慢病毒在原核系统和评估的诊断效用的纯化制备的间接ELISA的三种感染。结果表明，对于FIV和SRLV感染，CA和TM组分的组合增加了仅基于CA的诊断测试的灵敏度。与Coggins试验结果一致。(C)2004 Elsevier B.V.保留所有权利。
Among animal lentiviruses, Feline immunodeficiency virus (FIV), Equine infectious anaemia virus (EIAV) and Small ruminant lentiviruses (SRLV) are important pathogens associated with a variety of clinical pictures including immunodeficiency, anaemia, arthritis, pneumonia. The detection of viral antibody response represents a practical diagnostic approach in all lentivirus infections since they remain detectable long life. Capsid antigen (CA) is the major viral core protein and specific antibodies against this antigen are usually first recognised in infected sheep, goat and horse, remaining detectable for long period. Transmembrane (TM) domain of envelope glycoprotein contains a well conserved motif known to form an immunadominant epitope in several lentiviruses. In this study a simple strategy was developed to express the entire CA and the TM epitope in a single fusion protein from equine, feline and small ruminant lentiviruses in prokaryotic system and evaluated the diagnostic utility of a purified preparation in an indirect ELISA for each of the three infections. Results demonstrate that, for FIV and SRLV infections, the combination of CA and TM fractions increases the sensitivity of diagnostic tests based only on CA. The corresponding CA/TM antigen from EIAV showed excellent agreement with Coggins test. (C) 2004 Elsevier B.V. All rights reserved.