The self-orientation of mammalian cells in optical tweezers--the importance of the nucleus.
The self-orientation of mammalian cells in optical tweezers--the importance of the nucleus.
复制标题
光镊中哺乳动物细胞的自我定向——细胞核的重要性。
DOI：
10.1088/1478-3975/9/2/024001
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发表时间：
2012
期刊：
影响因子：
2
通讯作者：
Perney NM
中科院分区：
文献类型：
--
作者：
Perney NM
在这里，我们提出了第一个证据，表明真核细胞可以稳定地捕获在单个聚焦高斯光束中，其方向由细胞核定义。哺乳动物真核细胞（悬浮）被捕获并在腰部尺寸小于细胞核半径的线性偏振高斯光束的焦点中重新定向。细胞到达由细胞核和核成分决定的相对于焦点的位置。我们的研究表明，理论上可以预测光镊在细胞内位置施加的力；这样得到的数据与实验观察结果是一致的。
Here we present the first evidence showing that eukaryotic cells can be stably trapped in a single focused Gaussian beam with an orientation that is defined by the nucleus. A mammalian eukaryotic cell (in suspension) is trapped and is re-oriented in the focus of a linearly polarized Gaussian beam with a waist of dimension smaller than the radius of the nucleus. The cell reaches a position relative to the focus that is dictated by the nucleus and nuclear components. Our studies illustrate that the force exerted by the optical tweezers at locations within the cell can be predicted theoretically; the data obtained in this way is consistent with the experimental observations.