Construction of a shuttle vector for use between Pasteurella multocida and Escherichia coli.
Construction of a shuttle vector for use between Pasteurella multocida and Escherichia coli.
复制标题
构建用于多杀性巴氏杆菌和大肠杆菌之间的穿梭载体。
作者：
M. Bills;J. Medd;R. Chappel;B. Adler
从多杀性巴氏杆菌中分离小质粒，使得能够构建用于多杀性巴氏杆菌和大肠杆菌之间的穿梭载体。载体pBAC 64含有来自多杀性巴氏杆菌的复制起点、在多杀性巴氏杆菌中起作用的抗生素抗性基因和E.大肠杆菌载体pUC 18。pUC 18多克隆位点和lacZ ′基因的存在提供了一种筛选方法，并允许在E.大肠杆菌中的表达以及在多杀性巴氏杆菌中的克隆。
The isolation of a small plasmid from Pasteurella multocida has enabled the construction of a shuttle vector for use between P. multocida and Escherichia coli. The vector pBAC64 contains the origin of replication from P. multocida, an antibiotic resistance gene which functions in P. multocida, and the E. coli vector pUC18. The presence of the pUC18 multiple cloning site together with the lacZ' gene provides a screening method and allows cloning and manipulation in E. coli as well as cloning in P. multocida.