Growth and nodulation in Alnus sieboldiana in response to Frankia inoculation and nitrogen treatments

Growth and nodulation in Alnus sieboldiana in response to Frankia inoculation and nitrogen treatments
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西博尔桤木 (Alnus sieboldiana) 的生长和结瘤对 Frankia 接种和氮处理的反应

DOI:
10.1007/s00468-015-1246-8
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发表时间:
2016
期刊:
Tree
影响因子:
--
通讯作者:
Shingo KawaiTakashi
Shingo KawaiTakashi
中科院分区:
--
文献类型:
--
作者:
Takashi Yamanaka;Hiroaki Okabe;Shingo KawaiTakashi

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关键信息我们研究了Frankia-Alnussieboldiana共生,包括恒定结瘤的最小接种剂量、接种后到结瘤的时间以及氮对结瘤的影响。 摘要Frankia是一种固氮放线菌,在一些双子叶植物中形成根瘤,称为放线菌。我们研究了放线菌植物桤木(Alnus sieboldiana)接种弗兰奇分离物后的根瘤形成,以建立弗兰奇接种和接种植物的培养技术。 A. 幼苗上形成根瘤。接种后 2 周开始进行 sieboldiana,并在接种后 3 周开始通过乙炔还原活性测量 N2 固定。以 0.001 µL 细胞堆积体积 (pcv) 接种 aFrankiaisolate 后观察到结瘤。接种超过 0.05 µL pcv 的 Frankia 的幼苗上形成的根瘤数量没有显着差异。当每周用 15 mM NH4NO3-N 处理接种的幼苗时,根瘤发育和 N2 固定会减少。相比之下,在氮处理 15 周时,用 3.75 或 0.9375 mM NH4NO3-N 处理并不会抑制接种幼苗的根瘤发育或氮固定。
Key messageWe investigated aFrankia–Alnussieboldianasymbiosis, including the minimum inoculum dose for constant nodulation, the period of time to nodulation after inoculation, and the effects of N on nodulation.AbstractFrankiais a nitrogen-fixing actinomycete that forms root nodules in some dicotyledonous plants, which are called actinorhizal. We studied nodule formation inAlnus sieboldiana, an actinorhizal plant, after inoculation with aFrankiaisolate to establish techniques forFrankiainoculation and the cultivation of inoculated plants. Root nodules formed on seedlings ofA. sieboldianaby 2 weeks after inoculation, and N2fixation measured by acetylene reduction activity started 3 weeks after inoculation. Nodulation was observed after inoculation with aFrankiaisolate at 0.001 μL packed cell volume (pcv). The number of nodules formed on the seedlings inoculated withFrankiaat more than 0.05 μL pcv was not significantly different. Nodule development and N2fixation were reduced when inoculated seedlings were treated weekly with 15 mM NH4NO3-N. In contrast, treatment with 3.75 or 0.9375 mM NH4NO3-N did not inhibit nodule development or N2fixation of inoculated seedlings by 15 weeks of N treatment.
使用乙炔还原测定原位测量完整豆类共生体的固氮酶活性
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