Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde inhibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells
Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde inhibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells
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DOI：
10.1016/j.bbrc.2011.11.014
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发表时间：
2011-12-16
影响因子：
3.1
通讯作者：
Kadowaki, Makoto
中科院分区：
文献类型：
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作者：
Kageyama-Yahara, Natsuko;Wang, Xiaoyu;Kadowaki, Makoto
抗原IgE介导的粘膜肥大细胞活化在食物过敏的发展中至关重要。桂皮醛是桂皮的主要成分，可剂量依赖性地抑制抗原-IgE诱导的粘膜型骨髓源性肥大细胞（mBMMC）和RBL-2 H3细胞的脱颗粒。肉桂醛还抑制由抗原IgE刺激的mBMMC中细胞外Ca（2+）内流诱导的细胞内Ca（2+）水平的升高。此外，磷脂酶C（PLC）γ 1的酪氨酸磷酸化，这是一个关键的激活开关的细胞内Ca（2+）动员肥大细胞，被肉桂醛减弱。总之，我们的结果表明，肉桂醛抑制细胞内Ca（2+）动员和粘膜肥大细胞的脱粒抑制IgE受体PLC γ-Ca（2+）内流途径的活性。这些研究结果表明，肉桂醛可能具有治疗粘膜肥大细胞相关的过敏性疾病，如食物过敏的潜力。(C)2011 Elsevier Inc. All rights reserved.
Antigen-IgE-mediated mucosal mast-cell activation is critical in the development of food allergies. Cinnamaldehyde, a major constituent of Cinnamomi cortex, dose-dependently inhibited the antigen-IgE-induced degranulation of mucosal-type bone-marrow derived mast cells (mBMMCs) and RBL-2H3 cells. Cinnamaldehyde also suppressed the elevation of the intracellular Ca(2+) level that is induced by the extracellular Ca(2+) influx in antigen-IgE-stimulated mBMMCs. Furthermore, tyrosine phosphorylation of phospholipase C (PLC) gamma 1, which is a crucial activation switch for the intracellular Ca(2+) mobilization in mast cells, was attenuated by cinnamaldehyde. Together, our results demonstrated that cinnamaldehyde suppressed the intracellular Ca(2+) mobilization and the degranulation of mucosal mast cells by inhibiting the activity of the IgE receptor-PLC gamma-Ca(2+) influx pathway. These findings suggest that cinnamaldehyde may have therapeutic potential in mucosal mast cell-related allergic diseases, such as food allergies. (C) 2011 Elsevier Inc. All rights reserved.