PROMOTION OF RETINAL NEURITE OUTGROWTH BY SUBSTRATUM-BOUND FIBRONECTIN
PROMOTION OF RETINAL NEURITE OUTGROWTH BY SUBSTRATUM-BOUND FIBRONECTIN
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DOI：
10.1016/0012-1606(81)90328-6
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发表时间：
1981-01-01
影响因子：
2.7
通讯作者：
LILIEN, JE
中科院分区：
文献类型：
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作者：
AKERS, RM;MOSHER, DF;LILIEN, JE
在此条件下，鸡胚神经视网膜的聚集体将延长神经突起在体外进行了检查。用胰蛋白酶分散的7日龄鸡胚神经视网膜细胞在旋转培养中聚集8小时，并在各种标准培养基质上的无血清培养基中维持。聚集体在未处理的塑料、玻璃或胶原基质上延伸很少的神经突。用人血浆纤连蛋白预处理这些基质增强其支持视网膜神经突生长的能力。在纤连蛋白处理的基质上培养的聚集体在体外36小时内延伸出长的、放射状取向的神经突。这些神经突的形态是不同的，当聚合物培养在聚赖氨酸处理的基质。在后一种情况下，神经突是高度分支的，并且围绕聚集体周边同心地生长。纤维连接蛋白加入聚赖氨酸处理的基质刺激放射状神经突生长。促进神经突生长依赖于与培养基质结合的纤连蛋白的量和结合发生时的pH。纤维连接蛋白介导的神经突生长的要求比以前报道的成纤维细胞附着和扩散的要求更严格。
Conditions under which aggregates of embryonic chick neural retina will extend neurites in vitro were examined. Trypsin-dispersed cells from 7-day embryonic chick neural retinal were aggregated in rotation culture for 8 h and maintained in serum-free medium on a variety of standard culture substrata. Aggregates extend few neurites on untreated plastic, glass or collagen substrata. Pretreatment of these substrata with human plasma fibronectin enhances their capacity to support retinal neurite outgrowth. Aggregates cultured on fibronectin-treated substrata extend long, radially oriented neurites within 36 h in vitro. The morphology of these neurites is distinct from that seen when aggregates are cultured on polylysine-treated substrata. In the latter case neurites are highly branched and grow concentrically around the aggregate perimeter. Addition of fibronectin to polylysine-treated substrata stimulates radial neurite outgrowth. Promotion of neurite outgrowth is dependent on the amount of fibronectin bound to the culture substratum and on the pH at which binding occurs. The requirements for fibronectin-mediated neurite outgrowth are more stringent than those previously reported for fibroblast attachment and spreading.