Chemical Characterization of Klason Lignin Preparations from Plant-Based Foods
Chemical Characterization of Klason Lignin Preparations from Plant-Based Foods
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DOI：
10.1021/jf2031378
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发表时间：
2011-12-14
影响因子：
6.1
通讯作者：
Saha, Gerard Tchetseubu
中科院分区：
文献类型：
--
作者：
Bunzel, Mirko;Schuessler, Anne;Saha, Gerard Tchetseubu
为了分析Klason木质素方法用于测定植物基食品中木质素含量的准确性，对来自曲羽衣甘蓝、梨、全麦谷物和玉米麸皮的Klason木质素制剂进行了化学表征。表征包括常规灰分和蛋白质测定和脂肪/蜡的提取以及角质/木栓质解聚和释放单体的提取。通过重量分析法测定Klason木质素制剂中的脂肪/蜡和角质/木栓质的量，并且通过使用GC-MS分析它们的组成。（和木栓质）成分如饱和和不饱和脂肪酸、羟基和/或环氧脂肪酸和酚酸在所有样品中被鉴定，而长链烃、醇和酮、甾醇、甾烷醇，和二元酸依赖于分析的样品。非木质素化合物对Klason木质素含量的贡献的估计将不溶性纤维的未校正Klason木质素含量从28.7%（羽衣甘蓝）、22.8%（梨）、14.8%（小麦）和9.9%（玉米）降低到最大木质素含量6.5%（羽衣甘蓝）、16.4%（梨）、4.9%（小麦）和2.3%（玉米）。这些数据表明，某些常用的说法，如“谷物麸皮是高度ligands”需要修改。
To analyze the accuracy of the Klason lignin method as applied for the determination of lignin contents in plant based-food products, Klason lignin preparations from curly kale, pears, whole wheat grains, and corn bran were chemically characterized. Characterization included routine ash and protein determinations and the extraction of fat/waxes as well as cutin/suberin depolymerization and extraction of the liberated monomers. Fat/wax and cutin/suberin amounts in the Klason lignin preparations were determined gravimetrically, and their compositions were analyzed by using GC-MS. Typical fat, wax, and cutin (and suberin) constituents such as saturated and unsaturated fatty acids, hydroxy and/or epoxy fatty acids, and phenolic acids were identified in all samples, whereas the detection of long-chain hydrocarbons, alcohols, and ketones, sterols, stanols, and dioic acids was dependent on the sample analyzed. Estimation of the contribution of non-lignin compounds to the Klason lignin contents reduced the noncorrected Klason lignin contents of the insoluble fibers from 28.7% (kale), 22.8% (pear), 14.8% (wheat), and 9.9% (corn) to maximum lignin contents of 6.5% (kale), 16.4% (pear), 4.9% (wheat), and 2.3% (corn). These data demonstrate that certain commonly used statements such as "cereal brans are highly lignified" need to be revised.