A novel recombinant vaccine protecting mice against abrin intoxication.
A novel recombinant vaccine protecting mice against abrin intoxication.
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DOI：
10.1080/21645515.2015.1008879
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发表时间：
2015
影响因子：
4.8
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Wang J;Gao S;Xin W;Kang L;Xu N;Zhang T;Liu W;Wang J
由A链和B链组成的河豚毒素（AT）是一种潜在的生物恐怖因子，迫切需要一种有效的抗AT中毒疫苗。本研究成功地在大肠杆菌中表达了AT B链（AT B）。coli），并评估其对AT中毒的保护能力。重组ATB（rATB）亚基在4次免疫后诱导良好的免疫应答。所有BALB/c小鼠经腹膜内（i. p.）用5 × LD_（50）的AT攻击rATB蛋白的小鼠，小鼠存活。输注来自免疫小鼠的血清在幼稚小鼠中提供被动保护。此外，组织学结果表明，免疫rATB降低了毒素相关的组织损伤的严重程度。这项工作表明，rATB蛋白可能是一个有前途的疫苗候选人对人类暴露于AT。
Abrin toxin (AT) consisting of an A chain and a B chain is a potential agent for bioterrorism and an effective vaccine against AT poisoning is urgently required. In this study, AT B chain (ATB) was successfully expressed in the Escherichia coli (E. coli) and assessed the protection capacity against AT intoxication. The recombinant ATB (rATB) subunit induces a good immune response after 4 immunizations. All BALB/c mice immunized intraperitoneally (i.p.) with the purified rATB protein survived after challenged with 5 × LD50 of AT. Transfusion of sera from immunized mice provided passive protection in naive mice. Furthermore, histological findings showed that immunization with rATB decreased the severity of toxin-related tissue damage. This work indicates that the rATB protein may be a promising vaccine candidate against human exposure to AT.