Highly sensitive and specific bioassay for measuring bioactive TGF-beta.
Highly sensitive and specific bioassay for measuring bioactive TGF-beta.
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用于测量生物活性TGF-β的高度敏感和特异性生物测定。
DOI：
10.1186/1471-2121-7-15
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发表时间：
2006-03-20
期刊：
影响因子：
--
通讯作者：
Wyss-Coray T
中科院分区：
文献类型：
--
作者：
Tesseur I;Zou K;Berber E;Zhang H;Wyss-Coray T
Transforming Growth Factor-β (TGF-β) regulates key biological processes during development and in adult tissues and has been implicated in many diseases. To study the biological functions of TGF-β, sensitive, specific, and convenient bioassays are necessary. Here we describe a new cell-based bioassay that fulfills these requirements. Embryonic fibroblasts from Tgfb1-/- mice were stably transfected with a reporter plasmid consisting of TGF-β responsive Smad-binding elements coupled to a secreted alkaline phosphatase reporter gene (SBE-SEAP). Clone MFB-F11 showed more than 1000-fold induction after stimulation with 1 ng/ml TGF-β1, and detected as little as 1 pg/ml TGF-β1. MFB-F11 cells were highly induced by TGF-β1, TGF-β2 and TGF-β3, but did not show induction with related family members activin, nodal, BMP-2 and BMP-6 or with trophic factors bFGF and BDNF. MFB-F11 cells can detect and quantify TGF-β in biological samples without prior enrichment of TGF-βs, and can detect biologically activated TGF-β in a cell co-culture system. MFB-F11 cells can be used to rapidly and specifically measure TGF-β with high sensitivity.
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影响因子：
2.9
作者：
ABE, M;HARPEL, JG;RIFKIN, DB
通讯作者：
RIFKIN, DB
影响因子：
15.9
作者：
Ostroukhova, M;Seguin-Devaux, C;Ray, A
通讯作者：
Ray, A
影响因子：
4.8
作者：
Yagi, K;Goto, D;Miyazono, K
通讯作者：
Miyazono, K
影响因子：
15.9
作者：
Pittet, JF;Griffiths, MJD;Sheppard, D
通讯作者：
Sheppard, D
DOI：
10.1073/pnas.1834070100
发表时间：
2003-09-02
影响因子：
11.1
作者：
Yang, YC;Piek, E;Böttinger, EP
通讯作者：
Böttinger, EP