Mitotic factors from mammalian cells: a preliminary characterization.
Mitotic factors from mammalian cells: a preliminary characterization.
复制标题
哺乳动物细胞有丝分裂因子：初步表征。
DOI：
--
复制
发表时间：
1979
期刊：
影响因子：
--
通讯作者：
Potu N. Rao
中科院分区：
文献类型：
--
作者：
Prasad S. Sunkara;David A. Wright;Potu N. Rao
本研究的目的是从有丝分裂的HeLa细胞的因子，可以诱导非洲爪蟾卵母细胞减数分裂成熟的初步表征。我们发现，这个因素是一个热不稳定，钙敏感，nondialysisable蛋白质的沉降值为4-5S。此外，没有新的蛋白质合成被发现需要这种有丝分裂因子诱导两栖类卵母细胞成熟。这些数据表明，涉及核膜破裂和染色体浓缩的因素与三种不同的现象，有丝分裂，减数分裂和过早的染色体浓缩，在不同的动物物种是非常相似的。
The objective of this study was the preliminary characterization of the factors from mitotic HeLa cells that can induce meiotic maturation in Xenopus laevis oocytes. We found that this factor is a heat-labile, Ca2+-sensitive, nondialyzable protein with a sedimentation value of 4-5S. Furthermore, no new protein synthesis was found to be required for this mitotic factor to induce maturation in the amphibian oocytes. These data suggest that the factors involved in the breakdown of nuclear membrane and the condensation of chromosomes that are associated with three different phenomena, mitosis, meiosis, and premature chromosome condensation, are very similar in different animal species.