Analysis of topoisomerase function in bacterial replication fork movement: Use of DNA microarrays
Analysis of topoisomerase function in bacterial replication fork movement: Use of DNA microarrays
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DOI：
10.1073/pnas.97.17.9419
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发表时间：
2000-08-15
影响因子：
11.1
通讯作者：
Cozzarelli, NR
中科院分区：
文献类型：
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作者：
Khodursky, AB;Peter, BJ;Cozzarelli, NR
我们使用大肠杆菌基因组的 DNA 微阵列来追踪同步细胞中染色体复制叉的进展。我们发现 DNA 旋转酶和拓扑异构酶 IV (topo IV) 都促进复制叉的进展。当两种酶都被抑制时。复制叉迅速停止。单独使用拓扑IV的伸长率是正常的1/3。遗传数据证实并扩展了这些结果。单独的旋转酶失活会导致复制缓慢停止。 Topo IV 活性足以防止体内质粒 DNA 中 (+) 大结肠的积累，表明 topo IV 可以通过去除染色体叉前的 (+) 大结肠来促进复制。
We used DNA microarrays of the Escherichia coli genome to trace the progression of chromosomal replication forks in synchronized cells. We found that both DNA gyrase and topoisomerase IV (topo IV) promote replication fork progression. When both enzymes were inhibited. the replication fork stopped rapidly. The elongation rate with topo IV alone was 1/3 of normal. Genetic data confirmed and extended these results. Inactivation of gyrase alone caused a slow stop of replication. Topo IV activity was sufficient to prevent accumulation of (+) supercolis in plasmid DNA in vivo, suggesting that topo IV can promote replication by removing (+) supercolis in front of the chromosomal fork.