Diversity and distribution of sulphate-reducing bacteria in human faeces from healthy subjects and patients with inflammatory bowel disease
Diversity and distribution of sulphate-reducing bacteria in human faeces from healthy subjects and patients with inflammatory bowel disease
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DOI：
10.1111/j.1574-695x.2012.00935.x
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发表时间：
2012-06-01
影响因子：
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通讯作者：
Cole, Jeffrey A.
中科院分区：
文献类型：
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作者：
Jia, Wenjing;Whitehead, Rebekah N.;Cole, Jeffrey A.
比较了克罗恩病（CD）、肠易激综合征（IBS）或溃疡性结肠炎（UC）患者治疗前后粪便DNA中不同组硫酸盐还原菌（SRB）的相对丰度与健康对照组。生长试验显示，SRB并没有更丰富的CD患者的样品治疗前比健康对照组。对于128个可用样品中的大多数，使用扩增dsrAB基因的简并PCR引物证实了这些初步结果。然而，治疗前CD患者的一些样本含有IBS或UC样本中不存在的生长抑制剂。对PCR产生的dsrB片段进行的深入测序显示，检测到的多样性令人惊讶地低，只有8株SRB和亚硫酸盐还原菌Bilophila wadsaccharia检测到高于0.1%的阈值。在健康对照组中，检出的两种主要菌种，即类似于韦氏弧菌和脱硫弧菌的B，占总SR B菌群的比例高达93.5%，而在所有患者组中均较低。发现了四种以前未描述的物种：无法预测它们是硫酸盐还是亚硫酸盐还原菌。
The relative abundance of different groups of sulphate-reducing bacteria (SRB) in faecal DNA collected before and after therapy from patients suffering from Crohn's disease (CD), irritable bowel syndrome (IBS) or ulcerative colitis (UC) has been compared with that from healthy controls. Growth tests revealed that SRB were not more abundant in samples from patients with CD before treatment than in the healthy control group. For most of the 128 samples available, these preliminary results were confirmed using degenerate PCR primers that amplify the dsrAB gene. However, some samples from patients with CD before treatment contained a growth inhibitor that was absent from IBS or UC samples. In-depth sequencing of PCR-generated dsrB fragments revealed that the diversity detected was surprisingly low, with only eight strains of SRB and the sulphite-reducing bacterium, Bilophila wadsworthia, detected above the 0.1% threshold. The proportion of the two major species detected, B.similar to wadsworthia and Desulfovibrio piger, was as high as 93.5% of the total SRB population in the healthy control group and lower in all patient groups. Four previously undescribed species were found: it is impossible to predict whether they are sulphate or sulphite-reducing bacteria.