Xrs2p regulates Mre11p translocation to the nucleus and plays a role in telomere elongation and meiotic recombination
Xrs2p regulates Mre11p translocation to the nucleus and plays a role in telomere elongation and meiotic recombination
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DOI：
10.1097/mbc.e04-09-0782
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发表时间：
2005-02-01
影响因子：
3.3
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中科院分区：
文献类型：
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Mre 11-Rad 50-Xrs 2（MRX）蛋白复合物在减数分裂重组、受损DNA修复、端粒延长和细胞周期检查点控制中起关键作用。已知Xrs 2 p对复合物的所有功能都是必不可少的，但其在复合物中的作用尚未明确阐明。Xrs 2 p的C末端附近的32个氨基酸区域被鉴定为Mre 11 p结合位点。Xrs 2 p的功能并不比Mre 11 p从细胞质易位到细胞核对于响应DNA损伤是必需的。然而，除了32个氨基酸的区域外，Xrs 2 p中位于Mre 11 p结合位点上游49个氨基酸和下游104个氨基酸的结构域分别是减数分裂重组和端粒延伸所需的。这些研究结果表明，Xrs 2 p作为一个特异性因子，允许MRX复合物在减数分裂重组和端粒延长功能。
The Mre11-Rad50-Xrs2 (MRX) protein complex plays pivotal roles in meiotic recombination, repair of damaged DNA, telomere elongation, and cell cycle checkpoint control. Xrs2p is known to be essential for all the functions of the complex, but its role in the complex has not been clearly elucidated. A 32-amino acid region near the C terminus of Xrs2p was identified as an Mre11p-binding site. No more function of Xrs2p than translocation of Mre11p from the cytoplasm to the nucleus is necessary for response to DNA damage. However, domains in Xrs2p located both 49 amino acids upstream and 104 amino acids downstream of the Mre11p binding site are required for meiotic recombination and telomere elongation, respectively, in addition to the 32-amino acid region. These findings demonstrate that Xrs2p acts as a specificity factor that allows the MRX complex to function in meiotic recombination and in telomere elongation.