High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells
High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells
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DOI：
10.1263/jbb.103.98
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发表时间：
2007-01-01
影响因子：
2.8
通讯作者：
Wakitani, Shigeyuki
中科院分区：
文献类型：
--
作者：
Takagi, Mutsumi;Umetsu, Yousuke;Wakitani, Shigeyuki
研究了人骨髓间充质干细胞（MSCs）在添加地塞米松、TGF β 3和IGF-1的分化培养基中的密度对其向软骨细胞分化的影响，以用于软骨的再生治疗。在分化培养7 d时，随着MSCs初始密度从0.05 × 10 ~ 4/cm ~ 2增加到0.9 × 10 ~ 4/cm ~ 2，聚集蛋白聚糖mRNA表达水平增加。从分化培养物中收集的第7天的条件培养基（初始MSC密度为0.3 × 10（4）个细胞/cm（2））在分化培养物中加速了表达水平的初始增加，持续3天，其中分化培养物的初始MSC密度为0.3 × 10（4）个细胞/cm（2），而在分化培养的第7天收获的条件培养基中，初始MSC密度为0.05 × 10（4）个细胞/cm（2），则没有。14天后，以0.3 × 10（4）细胞/cm（2）的初始MSC浓度进行的分化培养显示出的表达水平是以0.05 × 10（4）细胞/cm（2）的初始MSC浓度进行的培养的表达水平的1.7倍。因此，高MSC接种密度可能适合于MSC向软骨细胞的快速分化。
The effects of the density of human mesenchymal stem cells (MSCs) on their differentioation to chondrocytes in a differentiation medium supplemented with dexamethasone, TGF beta 3, and IGF-1 were investigated for the regenerative therapy of cartilage. The increase in the initial density of MSCs from 0.05x10(4) to 0.9x10(4) cells/cm(2) accelerated the increase in the expression level of aggrecan mRNA during the differentiation culture for 7 d. The conditioned medium harvested at 7 d from the differentiation culture with an initial MSC density of 0.3 x 10(4) cells/cm(2) accelerated the initial increase in the expression level for 3 d in the differentiation culture with an initial MSC density of 0.3 x 10(4) cells/cm(2), whereas the conditioned medium harvested at 7 d in the differentiation culture with an initial MSC density of 0.05x10(4) cells/cm(2) did not. The differentiation culture after 14 d with an initial MSC concentration of 0.3 x 10(4) cells/cm(2) showed an expression level 1.7-fold that in the case of the culture with an initial MSC concentration of 0.05x10(4) cells/cm(2). Thus, a high MSC inoculum density might be appropriate for the rapid differentiation of MSCs to chondrocytes.