Construction and characterization of antibacterial PLGA/wool keratin/ornidazole composite membranes for periodontal guided tissue regeneration
Construction and characterization of antibacterial PLGA/wool keratin/ornidazole composite membranes for periodontal guided tissue regeneration
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牙周引导组织再生抗菌PLGA/羊毛角蛋白/奥硝唑复合膜的构建及表征
DOI：
10.1177/0885328220901396
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发表时间：
--
影响因子：
2.9
通讯作者：
Liu Jinsong
中科院分区：
文献类型：
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作者：
Zhang Hualin;Ma Hairong;Zhang Rui;Wang Kairong;Liu Jinsong
由细菌引起的术后感染现在被认为是引导组织再生（GTR）失败的主要原因。本研究采用静电纺丝法制备了6组负载抗菌剂奥硝唑的PLGA/羊毛角蛋白复合GTR膜（0% ORN、1% ORN、5% ORN、10% ORN、20% ORN、30% ORN复合膜）。测定复合膜的表面形貌和理化性质，评价复合膜的体外药物释放行为、抑菌性能和体外细胞毒性，以优化复合膜的载药量。含有1% ORN的复合膜具有较强的吸水性能和力学性能，且具有良好的体外降解和药物释放特性；5% ORN复合膜具有良好的吸水和降解性能；10% ORN复合膜具有良好的力学性能和降解性能。5种PLGA/羊毛角蛋白/奥硝唑复合膜可连续释放奥硝唑7 d。当复合膜中奥硝唑含量大于或等于10%时，可抑制人牙周韧带成纤维细胞（hPDLFs）的生长，而奥硝唑含量小于10%的复合膜可促进hPDLFs的生长和增殖，并促进其成骨分化。5种PLGA/羊毛角蛋白/奥硝唑复合膜均能抑制牙龈卟啉单胞菌（Pg）、核梭菌（Fn）和厌氧胃链球菌（Pa）的生长，且药物含量越高抑菌效果越强。综上所述，1% ORN复合膜具有良好的理化性能、生物相容性和抑菌性能，可作为抗菌GTR膜用于牙周组织修复。
Postoperative infections caused by bacteria are now thought to be the main cause of guided tissue regeneration (GTR) failure. In this study, six groups of PLGA/wool keratin composite GTR membranes loaded with the antibacterial agent ornidazole (0% ORN, 1% ORN, 5% ORN, 10% ORN, 20% ORN, and 30% ORN composite membranes) were prepared by an electrospinning method. The surface morphology and physicochemical properties of the composite membranes were determined, and the in vitro drug release behavior, bacteriostatic properties and in vitro cytotoxicity were assessed to optimize the amount of drug loaded into the composite membranes. The composite membrane with 1% ORN showed strong water absorption and mechanical properties as well as suitable in vitro degradation and drug release characteristics; the 5% ORN composite membrane showed good water absorption and degradation characteristics; and the 10% ORN composite membrane showed good mechanical and degradation properties. The five PLGA/wool keratin/ornidazole composite membranes could release ornidazole continuously for 7 d. When the content of ornidazole in the composite membranes was greater than or equal to 10%, the growth of human periodontal ligament fibroblasts (hPDLFs) was inhibited, while composite membranes with an ornidazole content of less than 10% facilitated the growth and proliferation of hPDLFs and could promote their osteogenic differentiation. The five PLGA/wool keratin/ornidazole composite membranes all inhibited the growth of Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn) and Peptostreptococcus anaerobius (Pa), and higher drug contents resulted in stronger bacteriostatic effects. In summary, the 1% ORN composite membrane had good physicochemical properties, biocompatibility and bacteriostatic properties, making this membrane applicable for use as an antibacterial GTR membrane for periodontal tissue repair.