Increased VH 11 and VH Q52 gene use by splenic B cells in old mice associated with oligoclonal expansions of CD5+B cells
Increased VH 11 and VH Q52 gene use by splenic B cells in old mice associated with oligoclonal expansions of CD5+B cells
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DOI：
10.1016/s0047-6374(98)00004-9
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发表时间：
1998-06-15
影响因子：
5.3
通讯作者：
Weksler, ME
中科院分区：
文献类型：
--
作者：
Ben-Yehuda, A;Szabo, P;Weksler, ME
与年轻小鼠相比，在LPS刺激的老年小鼠脾B淋巴细胞中观察到V-H基因家族VH 11和Q52的利用率显著增加。用V-H家族特异性和κ链探针通过原位DNA/RNA杂交测定V-H基因的使用。观察到的年龄依赖性差异似乎反映了CD 5 + B淋巴细胞亚群优先使用VH 11和Q52 V-H基因，其脾脏中的数量随年龄增加而增加。老年小鼠脾细胞对VH 11的使用增加与脾CD 5 + B淋巴细胞的克隆扩增有关。(C)1998由Elsevier Science爱尔兰有限公司出版。保留所有权利。
A significant increase in the utilization of the V-H gene families VH11 and Q52 was observed in LPS-stimulated splenic B lymphocytes from aged mice compared to young mice. V-H gene usage was assayed by in situ DNA/RNA hybridization using V-H family-specific and kappa chain probes. The observed age-dependent differences appear to reflect the preferential use of VH11 and Q52 V-H gene use by the CD5 + B lymphocyte subset whose numbers in the spleen increase with age. The increased use of VH11 by splenic cells from old mice is associated with clonal expansions of splenic CD5 + B lymphocytes. (C) 1998 Published by Elsevier Science Ireland Ltd. All rights reserved.