Coherent Raman Scattering Microscopy in Biology and Medicine.
Coherent Raman Scattering Microscopy in Biology and Medicine.
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DOI：
10.1146/annurev-bioeng-071114-040554
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发表时间：
2015
影响因子：
9.7
通讯作者：
Cheng JX
中科院分区：
文献类型：
--
作者：
Zhang C;Zhang D;Cheng JX
相干拉曼散射 (CRS) 显微镜的进步使得生命系统中功能性内源生物分子的无标记可视化和分析成为可能。与自发拉曼显微镜相比，CRS 显微镜的一个关键优势是成像速度的显着提高，从而可以对活体生物样品进行实时振动成像。最近开发的高光谱 CRS 显微镜利用分子振动特征，改进了 CRS 图像中化学信息的读取。在本文中，我们回顾了 CRS 显微技术的最新成就，重点介绍了 CRS 信噪比理论、成像速度、技术发展以及 CRS 成像在生物科学和临床环境中的应用。此外，我们还提出了该技术的使用未来可能的方向。
Advancements in coherent Raman scattering (CRS) microscopy have enabled label-free visualization and analysis of functional, endogenous biomolecules in living systems. When compared with spontaneous Raman microscopy, a key advantage of CRS microscopy is the dramatic improvement in imaging speed, which gives rise to real-time vibrational imaging of live biological samples. Using molecular vibrational signatures, recently developed hyperspectral CRS microscopy has improved the readout of chemical information available from CRS images. In this article, we review recent achievements in CRS microscopy, focusing on the theory of the CRS signal-to-noise ratio, imaging speed, technical developments, and applications of CRS imaging in bioscience and clinical settings. In addition, we present possible future directions that the use of this technology may take.