Early innate immune response and redistribution of inflammatory cells in the bony fish gilthead seabream experimentally infected with Vibrio anguillarum
Early innate immune response and redistribution of inflammatory cells in the bony fish gilthead seabream experimentally infected with Vibrio anguillarum
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DOI：
10.1007/s00441-004-1063-7
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发表时间：
2005-04-01
影响因子：
3.6
通讯作者：
Meseguer, J
中科院分区：
文献类型：
--
作者：
Chaves-Pozo, E;Muñoz, P;Meseguer, J
鱼类和哺乳动物的免疫系统之间的一个明显区别是，鱼类缺乏骨髓和淋巴结;在它们的位置，头肾作为成鱼的主要造血和淋巴器官，而胸腺，脾脏和粘膜相关的淋巴组织是鱼类和哺乳动物共有的。这表明抗原呈递和幼稚淋巴细胞刺激存在差异，这是启动适应性免疫应答的先决条件。以金头鲷（Sparus aurata L.）用完整的鳗弧菌作为颗粒细菌抗原，导致头肾白细胞向腹膜腔的移动，并引发它们的呼吸爆发活性。我们还观察到嗜酸性粒细胞（一种功能等同于哺乳动物中性粒细胞的白细胞）快速浸润到脾脏中。这些细胞可能参与抗原捕获和运输到脾脏，因为在该器官中已观察到动员的嗜酸性粒细胞、细菌抗原和增殖淋巴细胞之间的明显关联。总的来说，这些结果表明，除了积极参与细菌清除，鱼吞噬粒细胞在启动和支持的适应性免疫反应中发挥作用。
An obvious difference between the immune system of fish and mammals is that fish lack both bone marrow and lymph nodes; in their place, the head-kidney acts as a major haematopoietic and lymphoid organ in adult fish, whereas the thymus, spleen and mucosa-associated lymphoid tissues are common to both fish and mammals. This suggests that differences exist in antigen presentation and naive lymphocyte stimulation, a prerequisite for the initiation of adaptive immune responses. Intraperitoneal injection of the bony fish gilthead seabream (Sparus aurata L.) with intact Vibrio anguillarum, as a particulate bacterial antigen, results in the mobilisation of head-kidney leucocytes to the peritoneal cavity and priming of their respiratory burst activity. We have also observed the rapid infiltration of acidophilic granulocytes, which are leucocytes functionally equivalent to mammalian neutrophils, into the spleen. These cells may be involved in antigen capture and transport to the spleen, since an apparent association between mobilised acidophilic granulocytes, bacterial antigens and proliferating lymphocytes has been seen in this organ. Collectively, these results suggest that, in addition to being actively involved in bacterial clearance, fish phagocytic granulocytes play a role in the initiation and support of the adaptive immune response.