Fetal RHD genotyping in maternal serum during the first trimester of pregnancy
Fetal RHD genotyping in maternal serum during the first trimester of pregnancy
复制标题
妊娠前三个月母体血清中胎儿 RHD 基因分型
DOI：
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复制
发表时间：
2002
影响因子：
6.5
通讯作者：
E. Gautier
中科院分区：
文献类型：
--
作者：
Jean‐Marc Costa;Y. Giovangrandi;P. Ernault;L. Lohmann;V. Nataf;Najua El Halali;E. Gautier
摘要： 胎儿RHD基因检测在治疗致敏的RhD阴性孕妇中是有用的。妊娠早期可通过绒毛取样(CVS)或羊膜穿刺术确诊。然而，这些程序是侵入性的，导致胎儿丢失的风险增加，以及由于母胎出血而导致免疫接种的严重程度增加。本文报道了妊娠早期孕妇血清中胎儿DNA分析用于RHD基因分型的方法。在妊娠早期采集106份RhD阴性孕妇血清。这些血清被用一种新的实时聚合酶链式反应检测RHD基因的存在，并将结果与妊娠后期羊水细胞检测结果和新生儿RHD血清学结果进行比较。所有携带RhD阳性胎儿(n = 62)的孕妇血清RHD基因检测均为阳性，而携带RhD阴性胎儿(n = 40)的孕妇血清均为阴性。这项研究中获得的胎儿RHD基因分型的高准确度使这项技术能够在妊娠早期为RhD阴性患者提供常规治疗。
Summary. Fetal RHD genotype determination is useful in the management of sensitized RhD‐negative pregnant women. It can be ascertained early during pregnancy by chorionic villus sampling (CVS) or amniocentesis. However, these procedures are invasive, resulting both in an increased risk of fetal loss and in an increased severity of immunization due to fetomaternal haemorrhage. A reliable determination of RHD genotype by fetal DNA analysis in maternal serum during the first trimester of pregnancy is reported in this study. One hundred and six sera from RhD‐negative pregnant women were obtained during the first trimester of pregnancy. These sera were tested for the presence of RHD gene using a new real‐time polymerase chain reaction assay and the results compared with those obtained later in pregnancy on amniotic fluid cells and by RHD serology of the new‐born. All sera from women carrying a RhD‐positive fetus (n = 62) gave positive results for RHD gene detection and sera from women carrying a RhD‐negative fetus (n = 40) were negative. The high level of accuracy of fetal RHD genotyping obtained in this study could enable this technique to be offered on a routine basis for the management of RhD‐negative patients during the first trimester of pregnancy.
宫颈粘液和母血中的胎儿细胞。
DOI：
10.1053/beog.1999.0106
发表时间：
2000
期刊：
Bailliere's best practice & research. Clinical obstetrics & gynaecology
影响因子：
--
作者：
Holzgreve,W;Hahn,S
通讯作者：
Hahn,S
影响因子：
2.7
作者：
Wittwer, CT;Ririe, KM;Balis, UJ
通讯作者：
Balis, UJ