The cellular landscape by cryo soft X-ray tomography.
The cellular landscape by cryo soft X-ray tomography.
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DOI：
10.1007/s12551-019-00567-6
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发表时间：
2019-07-04
影响因子：
--
通讯作者：
Pereiro, E
中科院分区：
文献类型：
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作者：
Groen, J;Conesa, J J;Pereiro, E
结构细胞生物学中的成像技术对于理解细胞组织和机制是必不可少的。在此框架中，冷冻软 X 射线断层扫描 (cryo-SXT) 是一种基于同步加速器的成像技术，用于以纳米空间分辨率桥接电子显微镜和可见光荧光来分析完整的冷冻保存细胞的超微结构。凭借其与物质的独特相互作用和高穿透深度，X 射线是获取高分辨率和定量信息的非常有用和补充的来源。在这篇综述中，我们正在阿尔巴同步加速器（西班牙）的米斯特拉尔光束线上详细阐述了一个典型的冷冻相关工作流程，目的是通过冷冻-SXT 提供细胞的制图描述，说明该技术为细胞特征、细胞器组织和特定结构细胞生物学研究中的特定事件的特定定位带来的可能性。
Imaging techniques in structural cell biology are indispensable to understand cell organization and machinery. In this frame, cryo soft X-ray tomography (cryo-SXT), a synchrotron-based imaging technique, is used to analyze the ultrastructure of intact, cryo-preserved cells at nanometric spatial resolution bridging electron microscopy and visible light fluorescence. With their unique interaction with matter and high penetration depth, X-rays are a very useful and complementary source to obtain both high-resolution and quantitative information. In this review, we are elaborating a typical cryo correlative workflow at the Mistral Beamline at the Alba Synchrotron (Spain) with the goal of providing a cartographic description of the cell by cryo-SXT that illustrates the possibilities this technique brings for specific localization of cellular features, organelle organization, and particular events in specific structural cell biology research.