Sites of Fgf signalling and perception during embryogenesis of the beetle Tribolium castaneum
Sites of Fgf signalling and perception during embryogenesis of the beetle Tribolium castaneum
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DOI：
10.1007/s00427-007-0192-x
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发表时间：
2008-04-01
影响因子：
2.4
通讯作者：
Schroeder, Reinhard
中科院分区：
文献类型：
--
作者：
Beermann, Anke;Schroeder, Reinhard
多细胞胚胎的发育取决于细胞间信号传导事件的协调。在众多的细胞信号传导途径中，成纤维细胞生长因子（FGF）参与胚胎发生过程中的重要过程，例如原肠胚形成和生长过程中的中胚层形成。在脊椎动物中，Fgf 超家族由 22 个家族成员组成，而在不太复杂的昆虫和蠕虫基因组中只包含很少的 FGF。在最近测序的盗盗甲虫基因组中，我们鉴定了代表三个亚科的四个 Fgf 家族成员。谷盗具有果蝇中不存在但脊椎动物中已知的 Fgf1 基因。通过对果蝇的系统发育分析和微同线分析，我们进一步将Tc-fgf 8归类为Pyramus和Thisbe（果蝇Fgf8基因）的祖先。生长区中的 Tc-fgf8 表达表明参与中胚层形成。在胚胎头部中，Tc-fgf8 的表达将大脑分为较大的前部区域和较小的后部区域。 Fgf Tc-branchless 在胚胎气管基板和各种腺样结构中表达。唯一的Tribolium Fgf 受体和接头分子Downstream-of-Fgfr 的表达模式与Tc-Fgf8 和Tc-bnl 基本一致。因此，与果蝇相反，赤拟谷盗中只有一种 Fgf 受体在不同组织中传导 Fgf 信号传导。我们的发现极大地增进了我们对昆虫 Fgf 信号进化的理解。
The development of multicellular embryos depends on coordinated cell-to-cell signalling events. Among the numerous cell-signalling pathways, fibroblast growth factors (FGFs) are involved in important processes during embryogenesis, such as mesoderm formation during gastrulation and growth. In vertebrates, the Fgf superfamily consists of 22 family members, whereas only few FGFs are contained in the less complex genomes of insects and worms. In the recently sequenced genome of the beetle Tribolium, we identified four Fgf family members representing three subfamilies. Tribolium has Fgf1 genes that are absent in Drosophila but known from vertebrates. By phylogenetic analysis and microsynteny to Drosophila, we further classify Tc-fgf 8 as an ancestor of pyramus and thisbe, the fly Fgf8 genes. Tc-fgf8 expression in the growth zone suggests an involvement in mesoderm formation. In the embryonic head, expression of Tc-fgf8 subdivides the brain into a larger anterior and a smaller posterior region. The Fgf Tc-branchless is expressed in the embryonic tracheal placodes and in various gland-like structures. The expression patterns of the only Tribolium Fgf receptor and the adaptor molecule Downstream-of-Fgfr are largely congruent with Tc-Fgf8 and Tc-bnl. Thus, in contrast to Drosophila, only one Fgf receptor canalises Fgf signalling in different tissues in Tribolium. Our findings significantly advance our understanding of the evolution of Fgf signalling in insects.