Reactive Oxygen Species and Sperm Cells
Reactive Oxygen Species and Sperm Cells
复制标题
活性氧和精子细胞
DOI：
10.5772/intechopen.73037
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Y. Yumura
中科院分区：
文献类型：
--
作者：
T. Takeshima;Shinnosuke Kuroda;Y. Yumura
许多男性因素不育的病例是特发性的，但30-40%的病例可能在他们的精液中有过多的活性氧（ROS）。内源性ROS的来源有白细胞和未成熟精子，外因多种多样。相反，精浆中含有多种抗氧化剂。低水平的ROS对受精过程至关重要，但过多的ROS会导致氧化应激，并可能对生殖能力产生有害影响，如膜脂质过氧化、精子脱氧核糖核酸断裂和细胞凋亡。为了更好地评价氧化应激，采用纯精液化学发光法测定ROS，并定量精浆中8-OH-2′-脱氧鸟苷和丙二醛含量。抗氧化电位通常用总抗氧化能力（TAC）测定。MiOXSYS分析仪测量的氧化还原电位是一种新颖、更简单、更快速、更便宜的氧化应激测量技术。为了减少氧化应激和改善临床结果，考虑了精子分类方法，生活方式的改变，缩短射精禁欲时间，以及口服抗氧化剂，精索静脉曲张切除术和睾丸精子提取等治疗。展望未来，蛋白质组学、代谢组学和基因组学仍是发展中的领域，有可能发现新的发现和高度敏感的生物标志物。
Many cases of male factor infertility are idiopathic, but 30–40% of cases may have excessive levels of reactive oxygen species (ROS) in their semen. The origins of endogenous ROS are leukocytes and immature spermatozoa, and external causes are various. On the contrary, seminal plasma contains various antioxidants. Low levels of ROS are essential for the fertilization process, but excessive levels of ROS lead to oxidative stress and can have harmful effects such as lipid peroxidation of a membrane, sperm deoxyribonucleic acid fragmentation, and apoptosis on the fertile capacity. In order to evaluate oxidative stress appropriately, ROS is measured by the chemiluminescence method with neat semen and quantification of 8-OH-2′-deoxyguanosine and malondialdehyde in seminal plasma. Antioxidant potential is often measured using total antioxidant capacity (TAC) assay. The oxidation-reduction potential measured by a MiOXSYS analyzer is a novel, easier, quicker, and less expensive technology to measure oxidative stress. In order to minimize oxidative stress and improve clinical outcomes, sperm-sorting methods, lifestyle modifications, shortening the ejaculatory abstinence, and treatments such as oral antioxidants, varicocelectomy, and testicular sperm extraction are taken into account. As a future prospect, proteomics, metabolomics, and genomics are still developing areas that have the potential to discover new findings and highly sensitive biomarkers.
影响因子：
6.1
作者：
Suganuma, R;Yanagimachi, R;Meistrich, ML
通讯作者：
Meistrich, ML
DOI：
10.1073/pnas.88.24.11003
发表时间：
1991-12-01
影响因子：
11.1
作者：
FRAGA, CG;MOTCHNIK, PA;AMES, BN
通讯作者：
AMES, BN