Isolated Chloroplast Division Machinery Can Actively Constrict After Stretching
Isolated Chloroplast Division Machinery Can Actively Constrict After Stretching
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DOI：
10.1126/science.1129689
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发表时间：
2006-09
期刊：
影响因子：
56.9
通讯作者：
Y. Yoshida;H. Kuroiwa;O. Misumi;K. Nishida;F. Yagisawa;T. Fujiwara;H. Nanamiya;F. Kawamura;T. Kuroiwa
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文献类型：
--
作者：
Y. Yoshida;H. Kuroiwa;O. Misumi;K. Nishida;F. Yagisawa;T. Fujiwara;H. Nanamiya;F. Kawamura;T. Kuroiwa
叶绿体分裂包括质体分裂、动力蛋白和FtsZ （PDF）环。我们从红藻merolae的叶绿体中分离出完整的超扭曲（或螺旋）和圆形PDF机械。在用光学镊子将单个完整的PDF机器拉伸到其原始长度的四倍后，它们自发地恢复到原始尺寸。动力释放的PDF机械不保留螺旋结构，不能拉伸。因此，除了挤压膜外，动力蛋白还可以通过分裂叶绿体时丝的滑动产生收缩的动力。
Chloroplast division involves plastid-dividing, dynamin, and FtsZ (PDF) rings. We isolated intact supertwisted (or spiral) and circular PDF machineries from chloroplasts of the red alga Cyanidioschyzon merolae. After individual intact PDF machineries were stretched to four times their original lengths with optical tweezers, they spontaneously returned to their original sizes. Dynamin-released PDF machineries did not retain the spiral structure and could not be stretched. Thus, dynamin may generate the motive force for contraction by filament sliding in dividing chloroplasts, in addition to pinching-off the membranes.