Carbon dots-silver nanoparticles fluorescence resonance energy transfer system as a novel turn-on fluorescent probe for selective determination of cysteine
Carbon dots-silver nanoparticles fluorescence resonance energy transfer system as a novel turn-on fluorescent probe for selective determination of cysteine
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DOI：
10.1016/j.jphotochem.2015.04.016
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发表时间：
2015-08-15
影响因子：
4.3
通讯作者：
Hallaj, Tooba
中科院分区：
文献类型：
--
作者：
Amjadi, Mohammad;Abolghasemi-Fakhri, Zahra;Hallaj, Tooba
在本文中，我们利用荧光光谱研究了由橙汁制备的碳点（C-dots）与银纳米粒子（AgNP）的相互作用。研究发现，由于荧光共振能量转移（FRET），AgNPs 可以有效地猝灭碳点的荧光。因此，引入了 C 点（作为供体）和 AgNP（作为受体）之间的新型 FRET 系统。此外，我们发现，由于半胱氨酸在 AgNP 上的竞争性吸附，即使是纳摩尔水平的半胱氨酸也可以恢复碳点的荧光。利用这一点设计了一种简单且选择性的方法，用于测定浓度范围为 6.0 至 300 nM 的半胱氨酸，检测限为 4.0 nM。用该方法测定人血浆和尿液样品中的半胱氨酸，结果满意。 (C) 2015 Elsevier B.V. 保留所有权利。
In this paper, we investigated the interaction of carbon dots (C-dots) prepared from orange juice with silver nanoparticles (AgNPs) using fluorescence spectroscopy. It was found that AgNPs efficiently quench the fluorescence of C-dots as a result of fluorescence resonance energy transfer (FRET). Thus, a novel FRET system between C-dots (as the donor) and AgNPs (as the acceptor) was introduced. Moreover, it was found that cysteine even at nanomolar levels could recover the fluorescence of C-dots due to the competitive adsorption of this compound onto AgNPs. This was exploited to design a simple and selective method for the determination of cysteine in the concentration range from 6.0 to 300 nM, with a detection limit of 4.0 nM. Cysteine was determined by this method in human plasma and urine samples with satisfactory results. (C) 2015 Elsevier B.V. All rights reserved.