Involvement of ROP6 and clathrin in nodulation factor signaling
Involvement of ROP6 and clathrin in nodulation factor signaling
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DOI：
10.1080/15592324.2015.1033127
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发表时间：
2015-07
影响因子：
2.9
通讯作者：
Chao Wang;Xiaoshu Xu;Z. Hong;Yong Feng;Zhongming Zhang
中科院分区：
文献类型：
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作者：
Chao Wang;Xiaoshu Xu;Z. Hong;Yong Feng;Zhongming Zhang
豆科植物百脉根和固氮细菌中索根瘤菌之间的共生关系导致根瘤的形成。该过程始于宿主根细胞表面的 NF 受体 (NFR) 对根瘤菌结瘤因子 (NF) 的识别。 NFR 识别后的下游信号级联尚未完全表征。我们最近从 L. japonicus 中鉴定出网格蛋白重链 1 (CHC1) 作为 NF 信号级联的潜在靶标。 CHC 是真核细胞网格蛋白介导的内吞作用 (CME) 中已知的核心成分。 CHC1基因在根瘤菌感染的根毛中高表达，CHC1蛋白存在于感染袋附近和沿着感染线膜的细胞质点状结构中。此外，CHC1 显性失活变异的表达或 CME 化学抑制剂的治疗会导致 NF 信号传导、根瘤菌感染和结瘤的表型受损。这些发现为未来旨在了解内吞作用在 NF 信号通路和根瘤菌感染中的作用的工作开辟了新的途径。
The symbiotic association between the legume Lotus japonicus and the nitrogen-fixing bacterium Mesorhizobium loti results in the formation of root nodules. This process begins with the recognition of the rhizobial nodulation factor (NF) by the NF receptors (NFR) at the cell surface of the host roots. The downstream signaling cascades after NFR recognition have not been fully characterized. We recently identified a clathrin heavy chain 1 (CHC1) from L. japonicus as a potential target of the NF signaling cascades. CHC is a known central component in the clathrin-mediated endocytosis (CME) in eukaryotic cells. The CHC1 gene was highly expressed in Rhizobium-infected root hairs and the CHC1 protein was present in cytoplasmic punctate structures near the infection pockets and along the infection thread membrane. Furthermore, expression of a dominant-negative variant of CHC1 or treatment with a chemical inhibitor of CME resulted in impaired phenotypes in the NF signaling, rhizobial infection and nodulation. These findings open a new avenue for future work aiming at understanding the role of endocytosis in NF signaling pathway and rhizobial infection.