Development of a disposable three-compartment microcell culture device for toxicokinetic study in humans and its preliminary evaluation-猫眼课题宝

用于人体毒代动力学研究的一次性三室微细胞培养装置的研制及初步评价

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2006

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作者：

H. Nakayama;H. Kimura;K. Komori;T. Fujii;Y. Sakai

For animal-free in vitro prediction of systemic toxicity in humans, one possible approach is multicompartmental micro-cell culture device (on-chip human) where important organs-derived cells are simultaneously cultured in a physiologically-relevant perfusion circuit using microfluidic technologies. We newly developed a disposable-type three-compartment micro-cell culture based on polydimethylsiloxane (PDMS) and small magnetic stirrer-based pumps. By changing several valves on the device, we can select two different perfusion modes, that is, independent perfusion of each compartment and entire perfusion over the device, so that, cells derived from different organs can first be cultured independently according to individual culture protocol and then they are connected for entire perfusion to mimic systemic toxicity. As a preliminary evaluation, we isolated and immobilized rat primary mature adipocytes in the device because it primary controls the distribution of hydrophobic chemicals. We isolated and immobilized rat primary mature adipocytes. Although the specific gravity of mature adipocytes is slightly lower than that of culture medium, they were successfully immobilized in a homogeneously dispersed manner in a 3D non-woven fabrics-based scaffold. As a model of toxic chemicals, distribution of fluoranthene over the device was continuously and non-invasively monitored by an image acquisition system in terms of its fluorescence, and we successfully observed specific accumulation of fluoranthene in the adipocyte-containing fat tissue compartment.