The bacteriophage t7 virion undergoes extensive structural remodeling during infection.
The bacteriophage t7 virion undergoes extensive structural remodeling during infection.
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DOI：
10.1126/science.1231887
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发表时间：
2013-02-01
期刊：
影响因子：
--
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Hu B;Margolin W;Molineux IJ;Liu J
吸附和基因组估计是细菌噬菌体生命周期的基础，但它们的分子机制尚未充分了解尾纤维仅在宿主细胞表面结合到宿主细胞表面后，将尾部折叠起来，并对称地延伸。导致插入的尾巴插入，该尾巴是射精后的DNA射血导管，弹出的噬菌体尾巴倒塌或拆卸，这允许重新密封感染的细胞膜。噬菌体感染期间中间。
Adsorption and genome ejection are fundamental to the bacteriophage life cycle, yet their molecular mechanisms are not well understood. We used cryo–electron tomography to capture T7 virions at successive stages of infection of Escherichia coli minicells at ~4-nm resolution. The six phage tail fibers were folded against the capsid, extending and orienting symmetrically only after productive adsorption to the host cell surface. Receptor binding by the tail triggered conformational changes resulting in the insertion of an extended tail, which functions as the DNA ejection conduit into the cell cytoplasm. After ejection, the extended phage tail collapsed or disassembled, which allowed resealing of the infected cell membrane. These structural studies provide a detailed series of intermediates during phage infection.
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