An Activity Which Restores Theta Toxin Activity in Some Theta Toxin‐Deficient Mutants of Clostridium perfringens
An Activity Which Restores Theta Toxin Activity in Some Theta Toxin‐Deficient Mutants of Clostridium perfringens
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恢复某些产气荚膜梭菌 Theta 毒素缺陷突变体的 Theta 毒素活性的活性
DOI：
10.1111/j.1348-0421.1992.tb02050.x
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发表时间：
1992
影响因子：
2.6
通讯作者：
Yasushi Higashi
中科院分区：
文献类型：
--
作者：
T. Imagawa;Yasushi Higashi
产气荚膜梭菌 a 组突变体缺乏 θ 毒素，但会向培养物上清液中释放一种可透析物质（“A 物质”），从而恢复 b 组突变体的 θ 毒素活性； b 组突变体在“A 物质”释放方面存在缺陷。 “A物质”活性出现在a组突变体的指数生长期，并在稳定期消失。 “物质 A”活性在 pH 5.0 和 0°C 时最稳定，甚至在前 5 小时内增加了三倍，但在接下来的 15 小时内逐渐下降。它在 0°C 的中性和较高 pH 值下迅速失活。
Group a mutants of Clostridium perfringens are deficient in theta toxin but release a dialyzable substance (“substance A”), which restores theta toxin activity to group b mutants, into a culture supernatant; group b mutants are defective in “substance A” release. “Substance A” activity appeared in the exponentially growing phase of group a mutants and disappeared in the stationary phase. “Substance A” activity was most stable at pH 5.0 and 0 C and even increased threefold in the first 5 hr, but gradually decreased during the following 15 hr. It was quickly inactivated at neutral and higher pHs at 0 C.